de Oliveira Fernando Nivaldo, Ferreira Suzete Cleusa, Nishiya Anna Shoko, Mendrone-Junior Alfredo, Batista Marjorie Vieira, Rocha Vanderson, Costa Silvia Figueiredo
Laboratório de Investigação Médica (LIM-49), Instituto de Medicina Tropical, Faculdade de Medicina, Universidade de São Paulo, São Paulo, SP, Brazil.
Departamento de Biologia Molecular, Fundação Pró-Sangue/Hemocentro de São Paulo, São Paulo, SP, Brazil.
Transfus Med. 2023 Oct;33(5):403-408. doi: 10.1111/tme.12987. Epub 2023 Aug 1.
Brazil has a high prevalence of arboviruses, especially Dengue (DENV), Zika (ZKV), and Chikungunya (CHKV).
To study the risk of DENV, ZKV, and CHKV transmission by blood components in the haematopoietic stem cell transplantation (HSCT) population.
Prospective cohort of HSCT recipients and donors performed at the Hospital das Clinicas da FMUSP, São Paulo-Brazil. Patients were evaluated by serology and RT-PCR for DENV, ZKV, and CHKV pre-transplantation and once a week until neutrophil grafting. In positive cases (positive RT-PCR and/or serology conversion), an investigation was carried out on the blood components that the patient received to evaluate the possibility of it being transfusion transmitted.
A total of 93 patients were included during the study period. The mean age was 52 years with a predominance of males (56.9%). We considered five (5.3%) DENV cases positive by seroconversion in our study. One patient had IgM seroconversion and the other four presented IgG seroconversion to DENV. In the investigation of the blood components, 145 individual samples were analysed. None of the investigated blood components showed a positive RT-PCR.
We observed a low prevalence of DENV, ZKV, and CHKV in HSCT donors and recipients by serology and RT-PCR, and no case of blood transfusion transmission by RT-PCR.
巴西虫媒病毒的流行率很高，尤其是登革热病毒（DENV）、寨卡病毒（ZKV）和基孔肯雅病毒（CHKV）。
研究造血干细胞移植（HSCT）人群中血液成分传播DENV、ZKV和CHKV的风险。
在巴西圣保罗市FMUSP临床医院对HSCT受者和供者进行前瞻性队列研究。在移植前通过血清学和逆转录聚合酶链反应（RT-PCR）对患者进行DENV、ZKV和CHKV评估，每周评估一次，直至中性粒细胞植入。对于阳性病例（RT-PCR阳性和/或血清学转换），对患者接受的血液成分进行调查，以评估其输血传播的可能性。
在研究期间共纳入93例患者。平均年龄为52岁，男性占主导（56.9%）。在我们的研究中，通过血清学转换确定了5例（5.3%）DENV阳性病例。1例患者IgM血清学转换，另外4例出现DENV IgG血清学转换。在对血液成分的调查中，分析了145份个体样本。所有调查的血液成分RT-PCR均未呈阳性。
通过血清学和RT-PCR，我们观察到HSCT供者和受者中DENV、ZKV和CHKV的流行率较低，且未发现RT-PCR检测到的输血传播病例。
