Mota Magaly Lima, Dos Santos Souza Marinho Robson, Duro Rodrigo Lopes Sanz, Hunter James, de Menezes Irwin Rose Alencar, de Lima Silva João Marcos Ferreira, Pereira Glaubervânio Leite Tavares, Sabino Ester Cerdeira, Grumach Anete, Diaz Ricardo Sobhie, do Socorro Lucena Maria, Komninakis Shirley Vasconcelos
Faculty of Medical of the ABC, Santo André, SP, 09060-870, Brazil.
Centro Universitário Dr. Leão Sampaio, Juazeiro do Norte, CE, 63040-405, Brazil.
BMC Infect Dis. 2021 Jul 24;21(1):704. doi: 10.1186/s12879-021-06401-3.
The co-circulation of types of arbovirus in areas where they are endemic increased the risk of outbreaks and limited the diagnostic methods available. Here, we analyze the epidemiological profile of DENV, CHIKV and ZIKV at the serological and molecular level in patients with suspected infection with these arboviruses in the city of Juazeiro do Norte, Ceará, Brazil.
In 2016, the Central Public Health Laboratory (LACEN) of Juazeiro do Norte received 182 plasma samples from patients who visited health facilities with symptoms compatible with arbovirus infection. The LACEN performed serological tests for detection of IgM/IgG to DENV and CHIKV. They then sent these samples to the Retrovirology Laboratory of the Federal University of São Paulo and Faculty of Medical of the ABC where molecular analyses to confirm the infection by DENV, ZIKV and CHIKV were performed. The prevalence of IgM/IgG antibodies and of infections confirmed by RT-qPCR were presented with 95% confidence interval.
In serologic analysis, 125 samples were positive for antibodies against CHIKV and all were positive for antibodies against DENV. A higher prevalence of IgG against CHIKV (63.20% with 95% CI: 45.76-70.56) than against DENV (95.05% with 95% CI: 78.09-98.12) was observed. When the samples were submitted to analysis by RT-qPCR, we observed the following prevalence: mono-infection by ZIKV of 19.23% (95% CI: 14.29-34.82) patients, mono-infection by CHIKV of 3.84% (95% CI: 2.01-5.44) and co-infection with ZIKV and CHIKV of 1.09% (95% CI: 0.89-4.56).
The serologic and molecular tests performed in this study were effective in analyzing the epidemiological profile of DENV, CHIKV and ZIKV in patients with suspected infection by these arboviruses in the city of Juazeiro do Norte, Ceará/Brazil.
在虫媒病毒流行地区，多种虫媒病毒共同传播增加了疫情爆发的风险，并限制了可用的诊断方法。在此，我们在巴西塞阿拉州北朱泽鲁市对疑似感染这些虫媒病毒的患者进行血清学和分子水平分析，以了解登革病毒（DENV）、基孔肯雅病毒（CHIKV）和寨卡病毒（ZIKV）的流行病学特征。
2016年，北朱泽鲁市中央公共卫生实验室（LACEN）接收了182份来自出现与虫媒病毒感染症状相符的患者的血浆样本。LACEN进行了检测DENV和CHIKV IgM/IgG的血清学检测。然后他们将这些样本送至圣保罗联邦大学逆转录病毒实验室和ABC医学院，在那里进行分子分析以确认是否感染DENV、ZIKV和CHIKV。IgM/IgG抗体的患病率以及经逆转录定量聚合酶链反应（RT-qPCR）确认的感染率以95%置信区间表示。
血清学分析中，125份样本对CHIKV抗体呈阳性，所有样本对DENV抗体均呈阳性。观察到抗CHIKV IgG的患病率（63.20%，95%置信区间：45.76 - 70.56）高于抗DENV IgG的患病率（95.05%，95%置信区间：78.09 - 98.12）。当样本进行RT-qPCR分析时，我们观察到以下患病率：ZIKV单一感染患者为19.23%（95%置信区间：14.29 - 34.82），CHIKV单一感染为3.84%（95%置信区间：2.01 - 5.44），ZIKV和CHIKV共同感染为1.09%（95%置信区间：0.89 - 4.56）。
本研究中进行的血清学和分子检测有效地分析了巴西塞阿拉州北朱泽鲁市疑似感染这些虫媒病毒的患者中DENV、CHIKV和ZIKV的流行病学特征。
