Chiarelli-Neto Vito M, de Aveiro Emelly, Bronzato Juliana D, Arruda-Vasconcelos Rodrigo, Louzada Lidiane M, Godoi Ederaldo P, Lopes Erica M, de-Jesus-Soares Adriana, Ferraz Caio C R, Almeida José F A, Marciano Marina A, Gomes Brenda P F A
Division of Endodontics, Department of Restorative Dentistry, Piracicaba Dental School - State University of Campinas - UNICAMP, Piracicaba, Brazil.
Aust Endod J. 2023 Dec;49(3):711-719. doi: 10.1111/aej.12784. Epub 2023 Aug 1.
The objective of this study was to investigate the presence of culturable bacteria, endotoxins (LPS) and lipoteichoic acid (LTA) levels in teeth with normal vital pulp (NVP) with intact crowns (IC) and those with coronal restoration (CR) limited to the enamel level. A total of 20 teeth indicated for endodontic treatment due to prosthetic reasons were selected. Samples were collected from the root canals. The levels of cultivable bacteria, LPS and LTA were assessed. Statistical analyses were performed at significance level set at 5%. None of the teeth presented microbial growth. In the IC group, the LPS levels were limited to the lowest concentration of LPS. On the contrary, higher LPS and LTA levels were detected in teeth with CR. It was concluded that teeth with NVP and IC were negative for bacteria, LPS and LTA; while teeth with CR were positive for bacterial virulence factors.
本研究的目的是调查具有完整牙冠（IC）的正常活髓（NVP）牙齿以及仅局限于釉质水平进行冠修复（CR）的牙齿中可培养细菌、内毒素（LPS）和脂磷壁酸（LTA）的水平。总共选择了20颗因修复原因而需进行牙髓治疗的牙齿。从根管中采集样本。评估可培养细菌、LPS和LTA的水平。在设定为5%的显著性水平上进行统计分析。所有牙齿均未出现微生物生长。在IC组中，LPS水平限于LPS的最低浓度。相反，在进行CR的牙齿中检测到更高的LPS和LTA水平。得出的结论是，具有NVP和IC的牙齿在细菌、LPS和LTA方面呈阴性；而进行CR的牙齿在细菌毒力因子方面呈阳性。
