Godoi Ederaldo P, Fagundes Pedro Ivo G, Francisco Priscila A, Delboni Maraísa G, Barbosa-Ribeiro Marlos, Endo Marcos S, Lopes Erica M, Martinho Frederico C, Gomes Brenda P F A
Department of Restorative Dentistry, Endodontics Division, Piracicaba Dental School, State University of Campinas-UNICAMP, Piracicaba, SP, Brazil.
School of Dentistry, Universidade de Pernambuco - Arcoverde, Pernambuco, PR, Brazil.
Aust Endod J. 2025 Aug;51(2):458-465. doi: 10.1111/aej.12963. Epub 2025 Jul 2.
This study aimed to quantify LPS and LTA levels in root canals requiring endodontic retreatment due to secondary or persistent infection and evaluate the relationship between Gram-positive and Gram-negative virulence factors and clinical symptoms and signs. Samples were collected from the root canals of 40 patients who needed endodontic retreatment and had radiographic evidence of apical periodontitis. Lipopolysaccharides (LPS) and lipoteichoic acid (LTA) were quantified using Limulus amebocyte lysate and enzyme-linked immunosorbent assay tests, respectively. LPS and LTA were detected in all samples, with mean values being 5.1 EU/mL(±2.83) and 537.53 pg/mL(±134.14), respectively. Significant correlations were found between levels of LPS and LTA with clinical features. In conclusion, coronal restoration directly influences the load of LPS and LTA in root canals with secondary infection. Furthermore, higher concentrations of endotoxin (LPS) are associated with larger areas of periapical bone resorption, as well as with the presence of clinical signs and symptoms.
本研究旨在量化因继发感染或持续性感染而需要进行根管再治疗的根管内脂多糖（LPS）和脂磷壁酸（LTA）水平，并评估革兰氏阳性和革兰氏阴性毒力因子与临床症状和体征之间的关系。样本取自40例需要进行根管再治疗且有根尖周炎影像学证据的患者的根管。分别使用鲎试剂法和酶联免疫吸附测定法对脂多糖（LPS）和脂磷壁酸（LTA）进行定量。所有样本中均检测到LPS和LTA，其平均值分别为5.1 EU/mL（±2.83）和537.53 pg/mL（±134.14）。发现LPS和LTA水平与临床特征之间存在显著相关性。总之，冠部修复直接影响继发感染根管内LPS和LTA的负荷。此外，较高浓度的内毒素（LPS）与较大面积的根尖周骨吸收以及临床症状和体征的存在有关。
