College of Plant Protection, Hebei Agricultural University, Baoding 071001, Hebei, China.
Baoding University, Baoding 071001, Hebei, China.
Plant Dis. 2024 Jan;108(1):13-19. doi: 10.1094/PDIS-01-23-0105-SR. Epub 2024 Jan 3.
Wheat leaf rust (), which is caused by Eriks. (), is one of the most important wheat diseases affecting wheat production globally. Using resistant wheat cultivars is the most economical and environmentally friendly way to control leaf rust. The Italian wheat cultivar Libellula has demonstrated good resistance to in field studies. To identify the genetic basis of resistance in 'Libellula', 248 F recombinant inbred lines from the cross 'Libellula'/'Huixianhong' was phenotyped for severity in seven environments: the 2014/2015, 2016/2017, 2017/2018, and 2018/2019 cropping seasons at Baoding, Hebei Province, and the 2016/2017, 2017/2018, and 2018/2019 crop seasons at Zhoukou, Henan Province. Bulked segregant analysis and simple sequence repeat markers were then used to identify the quantitative trait loci (QTLs) for adult-plant resistance in the population. Six QTLs were consequently detected and designated as and that were presumed to be new and , , , and that were identified at similar physical positions as previously reported QTLs. Based on chromosome positions and molecular marker tests, and share similar flanking markers with and , respectively. and are race nonspecific adult plant resistance (APR) genes for leaf rust and stripe rust and powdery mildew. showed multiple disease resistance to leaf rust, stripe rust, Fusarium head blight, and powdery mildew. The QTL identified in this study, as well as their closely linked markers, may potentially be used in marker-assisted selection in wheat breeding.
小麦叶锈病（），由 Eriks.（）引起，是影响全球小麦生产的最重要的小麦病害之一。使用抗性小麦品种是控制叶锈病最经济和环保的方法。意大利小麦品种 Libellula 在田间研究中表现出对 的良好抗性。为了鉴定 'Libellula'中 抗性的遗传基础，使用 248 个 'Libellula'/'Huixianhong'杂交的重组自交系在七个环境中对 严重度进行表型分析：2014/2015、2016/2017、2017/2018 和 2018/2019 作物季节在河北省保定市，以及 2016/2017、2017/2018 和 2018/2019 作物季节在河南省周口市。然后使用 bulked segregant analysis 和简单序列重复标记来鉴定群体中对成株期抗性的数量性状基因座 (QTL)。最终检测到六个 QTL，并将其命名为 和 ，这两个 QTL 被认为是新的，而 、 、 、 和 则与先前报道的 QTL 位于相似的物理位置。根据染色体位置和分子标记测试， 和 与 和 分别共享相似的侧翼标记。 和 是对叶锈病、条锈病和白粉病具有非专化性成株期抗性 (APR) 的基因。 表现出对叶锈病、条锈病、赤霉病和白粉病的多重抗性。本研究中鉴定的 QTL 及其紧密连锁标记可能潜在地用于小麦育种中的标记辅助选择。
