Lillemo M, Asalf B, Singh R P, Huerta-Espino J, Chen X M, He Z H, Bjørnstad A
Department of Plant and Environmental Sciences, Norwegian University of Life Sciences, P.O. Box 5003, 1432 As, Norway.
Theor Appl Genet. 2008 May;116(8):1155-66. doi: 10.1007/s00122-008-0743-1.
Powdery mildew, caused by Blumeria graminis f. sp. tritici is a major disease of wheat (Triticum aestivum L.) that can be controlled by resistance breeding. The CIMMYT bread wheat line Saar is known for its good level of partial and race non-specific resistance, and the aim of this study was to map QTLs for resistance to powdery mildew in a population of 113 recombinant inbred lines from a cross between Saar and the susceptible line Avocet. The population was tested over 2 years in field trials at two locations in southeastern Norway and once in Beijing, China. SSR markers were screened for association with powdery mildew resistance in a bulked segregant analysis, and linkage maps were created based on selected SSR markers and supplemented with DArT genotyping. The most important QTLs for powdery mildew resistance derived from Saar were located on chromosomes 7DS and 1BL and corresponded to the adult plant rust resistance loci Lr34/Yr18 and Lr46/Yr29. A major QTL was also located on 4BL with resistance contributed by Avocet. Additional QTLs were detected at 3AS and 5AL in the Norwegian testing environments and at 5BS in Beijing. The population was also tested for leaf rust (caused by Puccinia triticina) and stripe rust (caused by P. striiformis f. sp. tritici) resistance and leaf tip necrosis in Mexico. QTLs for these traits were detected on 7DS and 1BL at the same positions as the QTLs for powdery mildew resistance, and confirmed the presence of Lr34/Yr18 and Lr46/Yr29 in Saar. The powdery mildew resistance gene at the Lr34/Yr18 locus has recently been named Pm38. The powdery mildew resistance gene at the Lr46/Yr29 locus is designated as Pm39.
由小麦白粉菌引起的白粉病是小麦（普通小麦）的一种主要病害，可通过抗性育种加以控制。国际玉米小麦改良中心（CIMMYT）的面包小麦品系萨尔（Saar）以其良好的部分抗性和小种非特异性抗性水平而闻名。本研究的目的是在一个由萨尔与感病品系阿沃塞特（Avocet）杂交产生的113个重组自交系群体中，定位抗白粉病的数量性状位点（QTL）。该群体在挪威东南部的两个地点进行了为期两年的田间试验，并在中国北京进行了一次试验。在混合分离分析中筛选了与白粉病抗性相关的简单序列重复（SSR）标记，并基于选定的SSR标记构建了连锁图谱，同时补充了多样性微阵列分析（DArT）基因分型。源自萨尔的对白粉病抗性最重要的QTL位于7DS和1BL染色体上，与成株抗锈病基因座Lr34/Yr18和Lr46/Yr29相对应。一个主要的QTL也位于4BL上，其抗性由阿沃塞特贡献。在挪威的试验环境中，在3AS和5AL以及在北京的5BS上检测到了其他QTL。该群体还在墨西哥进行了叶锈病（由小麦叶锈菌引起）、条锈病（由小麦条锈菌引起）抗性以及叶尖坏死的测试。这些性状的QTL在与白粉病抗性QTL相同的位置7DS和1BL上被检测到，证实了萨尔中存在Lr34/Yr18和Lr46/Yr29。Lr34/Yr18基因座上的白粉病抗性基因最近被命名为Pm38。Lr46/Yr29基因座上的白粉病抗性基因被指定为Pm39。
