Liposome vesicle cannot be formed in non-aqueous phase.

In the February of 2023, Yang et al., report a paper to unreal mechanisms of protein and lipid oxidation in mayonnaise with claim of liposome formation in Mayonaise. As bilayer liposome has spherical or oval like structure composed bilayers separated by aqueous region, there is doubt for formation possibility of liposome in non-aqueous phase. In this bilayer vesicle a hydrophobic layer has been replaced between two outer and inner hydrophilic molecules, so regarding thermodynamic rules for tendency to a minimum enthalpy formation of bilayer vesicle with a hydrophobic outer layer in Mayonaise matrix is not possible. It seems more likely the micelle structure instead of liposome. The images in the article is indeed hard to see a liposomal bilayer structure. In other case, more explanation and evidences are required to clarify ambiguous points in this claim. So, arrangement of self-assembling of water soluble phospholipid may occur just in aqueous phase liposome formation is not possible. Also no document exists for bilayer of vesicles. So, those formed vesicle could be reverse micelle not liposome which usually form following dispersion of phospholipids in aqueous phase.