低氧增强水牛（Bubalus bubalis）颗粒细胞的甾体生成能力。
Hypoxia enhances steroidogenic competence of buffalo (Bubalus bubalis) granulosa cells.
机构信息
College of Animal Science and Technology, Guangxi Key Laboratory of Animal Breeding and Disease Control, Guangxi University, Nanning, 530005, China.
Laboratory Animal Center, Guangxi Medical University, Nanning, 530021, China.
出版信息
Theriogenology. 2023 Oct 15;210:214-220. doi: 10.1016/j.theriogenology.2023.07.031. Epub 2023 Jul 26.
Granulosa cells (GCs) synthesize estrogens needed for follicular growth. However, the effects of hypoxia on steroidogenesis in buffalo GCs remain unclear. In this study, the impacts of hypoxic conditions (5% oxygen) on estrogen synthesis in buffalo GCs were examined. The results showed that hypoxia improved both the expression levels of estrogen synthesis-related genes (CYP11A1, CYP19A1, and 3β-HSD) and the secretion levels of estradiol in buffalo GCs. Hypoxic conditions promoted the sensitivity of buffalo GCs to FSH. Furthermore, inhibition of cAMP/PKA signaling pathway (H89, a cAMP/PKA signaling pathway inhibitor) reduced both the expression levels of estrogen synthesis-related genes (CYP11A1, CYP19A1, and 3β-HSD) and the secretion levels of estradiol in hypoxia-cultured buffalo GCs. Besides, inhibition of cAMP/PKA signaling pathway lowered the responsiveness of buffalo GCs to FSH under hypoxic conditions. The present study indicated that hypoxia enhanced the steroidogenic competence of buffalo GCs principal by affecting cAMP/PKA signaling pathway and subsequent sensitivity of GCs to FSH.
颗粒细胞（GCs）合成卵泡生长所需的雌激素。然而，缺氧对水牛 GCs 类固醇生成的影响尚不清楚。在这项研究中，研究了低氧条件（5%氧气）对水牛 GCs 中雌激素合成的影响。结果表明，低氧条件可提高雌激素合成相关基因（CYP11A1、CYP19A1 和 3β-HSD）的表达水平和水牛 GCs 中雌二醇的分泌水平。低氧条件促进了水牛 GCs 对 FSH 的敏感性。此外，抑制 cAMP/PKA 信号通路（H89，cAMP/PKA 信号通路抑制剂）降低了低氧培养的水牛 GCs 中雌激素合成相关基因（CYP11A1、CYP19A1 和 3β-HSD）的表达水平和雌二醇的分泌水平。此外，抑制 cAMP/PKA 信号通路降低了低氧条件下 FSH 对水牛 GCs 反应性。本研究表明，缺氧通过影响 cAMP/PKA 信号通路以及随后 GCs 对 FSH 的敏感性，增强了水牛 GCs 的甾体生成能力。
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