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TRIM28 通过调节核受体信号通路来调节子宫功能。

TRIM28 modulates nuclear receptor signaling to regulate uterine function.

机构信息

Reproductive and Developmental Biology Laboratory, National Institute of Environmental Health Sciences, Research Triangle Park, NC, USA.

Integrative Bioinformatics, National Institute of Environmental Health Sciences, Research Triangle Park, NC, USA.

出版信息

Nat Commun. 2023 Aug 1;14(1):4605. doi: 10.1038/s41467-023-40395-7.

DOI:10.1038/s41467-023-40395-7
PMID:37528140
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10393996/
Abstract

Estrogen and progesterone, acting through their cognate receptors the estrogen receptor α (ERα) and the progesterone receptor (PR) respectively, regulate uterine biology. Using rapid immunoprecipitation and mass spectrometry (RIME) and co-immunoprecipitation, we identified TRIM28 (Tripartite motif containing 28) as a protein which complexes with ERα and PR in the regulation of uterine function. Impairment of TRIM28 expression results in the inability of the uterus to support early pregnancy through altered PR and ERα action in the uterine epithelium and stroma by suppressing PR and ERα chromatin binding. Furthermore, TRIM28 ablation in PR-expressing uterine cells results in the enrichment of a subset of TRIM28 positive and PR negative pericytes and epithelial cells with progenitor potential. In summary, our study reveals the important roles of TRIM28 in regulating endometrial cell composition and function in women, and also implies its critical functions in other hormone regulated systems.

摘要

雌激素和孕激素分别通过其同源受体雌激素受体 α（ERα）和孕激素受体（PR）发挥作用，调节子宫生物学功能。使用快速免疫沉淀和质谱（RIME）和共免疫沉淀技术，我们鉴定出 TRIM28（三肽重复含 28 个氨基酸）作为一种与 ERα 和 PR 相互作用的蛋白质，在调节子宫功能中发挥作用。TRIM28 表达的缺失导致子宫无法通过改变 PR 和 ERα 在子宫内膜上皮和基质中的作用来支持早期妊娠，这是通过抑制 PR 和 ERα 染色质结合来实现的。此外，在表达 PR 的子宫细胞中敲除 TRIM28 会导致具有祖细胞潜能的 TRIM28 阳性和 PR 阴性周细胞和上皮细胞的富集。总之，我们的研究揭示了 TRIM28 在调节女性子宫内膜细胞组成和功能中的重要作用，并暗示其在其他激素调节系统中的关键作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd98/10393996/6e8b84486a11/41467_2023_40395_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd98/10393996/1e5495ef34c6/41467_2023_40395_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd98/10393996/5d8d090413e9/41467_2023_40395_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd98/10393996/7c3248387970/41467_2023_40395_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd98/10393996/31789fc7ec65/41467_2023_40395_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd98/10393996/6c4e0257f00e/41467_2023_40395_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd98/10393996/e4fba130b7d2/41467_2023_40395_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd98/10393996/c58adec53f8a/41467_2023_40395_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd98/10393996/6da7e890a9d8/41467_2023_40395_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd98/10393996/6e8b84486a11/41467_2023_40395_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd98/10393996/1e5495ef34c6/41467_2023_40395_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd98/10393996/5d8d090413e9/41467_2023_40395_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd98/10393996/7c3248387970/41467_2023_40395_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd98/10393996/31789fc7ec65/41467_2023_40395_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd98/10393996/6c4e0257f00e/41467_2023_40395_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd98/10393996/e4fba130b7d2/41467_2023_40395_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd98/10393996/c58adec53f8a/41467_2023_40395_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd98/10393996/6da7e890a9d8/41467_2023_40395_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd98/10393996/6e8b84486a11/41467_2023_40395_Fig9_HTML.jpg

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2
MicroRNAs and Progesterone Receptor Signaling in Endometriosis Pathophysiology.微小 RNA 与孕激素受体信号在子宫内膜异位症发病机制中的作用。
Cells. 2022 Mar 24;11(7):1096. doi: 10.3390/cells11071096.
3
Progesterone Actions and Resistance in Gynecological Disorders.孕激素在妇科疾病中的作用及其抵抗机制。
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Commun Med (Lond). 2024 Dec 19;4(1):267. doi: 10.1038/s43856-024-00694-6.
4
Postnatal Ovarian Transdifferentiation in the Absence of Estrogen Receptor Signaling Is Dependent on Genetic Background.在缺乏雌激素受体信号的情况下，产后卵巢转分化依赖于遗传背景。
Endocrinology. 2024 Nov 26;166(1). doi: 10.1210/endocr/bqae157.
5
Generation of Oviductal Glycoprotein 1 Cre Mouse Model for the Study of Secretory Epithelial Cells of the Oviduct.输卵管糖蛋白 1 启动子 Cre 小鼠模型的建立及其在输卵管分泌上皮细胞研究中的应用。
Endocrinology. 2024 May 27;165(7). doi: 10.1210/endocr/bqae070.
6
A GREB1-steroid receptor feedforward mechanism governs differential GREB1 action in endometrial function and endometriosis.一种GREB1-类固醇受体前馈机制调控GREB1在子宫内膜功能和子宫内膜异位症中的不同作用。
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Cells. 2022 Feb 13;11(4):647. doi: 10.3390/cells11040647.
4
The role of epithelial progesterone receptor isoforms in embryo implantation.上皮孕酮受体亚型在胚胎着床中的作用。
iScience. 2021 Nov 20;24(12):103487. doi: 10.1016/j.isci.2021.103487. eCollection 2021 Dec 17.
5
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