脑肿瘤组织中 PDK1、SLC2A1、EGFR、PTEN、CD276 基因表达水平及 IDH1 基因 R132H 多态性的测定。

Determination of PDK1, SLC2A1, EGFR, PTEN, CD276 Gene Expression Levels and IDH1 Gene R132H Polymorphism in Brain Tumor Tissues.

机构信息

Afyonkarahisar Health Sciences University, Faculty of Medicine, Department of Neurosurgery, Afyonkarahisar, Turkey.

出版信息

Turk Neurosurg. 2023;33(6):1086-1092. doi: 10.5137/1019-5149.JTN.41102-22.6.

DOI:10.5137/1019-5149.JTN.41102-22.6

PMID:37528722

Abstract

AIM

To determine IDH1 R132H codon and the mRNA levels of PDK1, SLC2A1, EGFR, PTEN, and CD276 genes in brain tumors.

MATERIAL AND METHODS

This study included 15 brain tumor tissues [pituitary adenoma (1), pilocytic astrocytoma (1), mixed meningioma (2), mesothelial meningioma (2), atypical meningioma (1), immature teratoma (1), glioblastoma (4), meningioma (2), and bladder cancer metastasis (1)]. The expression levels of genes in brain tumor tissues were analyzed using real-time PCR. Sanger sequencing was performed to identify the IDH1 gene R132H codon.

RESULTS

All cases were wild-type in terms of IDH1 R132H: nucleotide 395 G > A; codon CGT > CAT. The mRNA level of PDK1 was lower in grade I tumor tissues (0.675-fold) and increased in grades II-III-IV (7.135, 16.912, and 7.081-fold, respectively) (p < 0.001). The mRNA level of SLC2A1 decreased in all grades I-II-III-IV [(0.424-, 0.093-, 0.234 (p < 0.001), and 0.141-fold (p < 0.005), respectively)]. The mRNA level of EGFR increased in all grades I-II-III-IV [1.388, 5.452 (p < 0.017), 4.624-, and 4.137-fold, respectively]. The mRNA level of PTEN increased in grades I-II-III [1.802-, 1.702-, and 1.5-fold, respectively] and decreased in grade IV (0.176-fold). The mRNA level of CD276 increased in all grades I-II-III-IV [1.8-, 5.756-(p < 0.001), 10.303 (p < 0.001), and 2.5-fold, respectively].

CONCLUSION

We obtained similar findings for previously reported PDK1, EGFR, PTEN, and CD276 gene expression levels. In contrast, SLC2A1 expression was markedly downregulated, as reported in other tumor studies. These findings may be due to the unique nature of brain tumor tissues. Additionally, a decrease in PTEN gene expression has been observed in grade IV brain tumors, including glioblastoma and meningioma. Although the size of the analyzed study group was limited, the gene expression results showed similarities in the behavior of genes during cancer staging.

摘要

目的

检测脑肿瘤中 IDH1 R132H 密码子和 PDK1、SLC2A1、EGFR、PTEN 和 CD276 基因的 mRNA 水平。

材料和方法

本研究纳入了 15 例脑肿瘤组织[垂体腺瘤（1）、毛细胞型星形细胞瘤（1）、混合脑膜瘤（2）、间皮脑膜瘤（2）、非典型脑膜瘤（1）、未成熟畸胎瘤（1）、胶质母细胞瘤（4）、脑膜瘤（2）和膀胱癌转移（1）]。采用实时 PCR 分析脑肿瘤组织中基因的表达水平。采用 Sanger 测序鉴定 IDH1 基因 R132H 密码子。

结果

所有病例 IDH1 R132H 均为野生型：核苷酸 395 G > A；密码子 CGT > CAT。I 级肿瘤组织中 PDK1 的 mRNA 水平降低（0.675 倍），II-III-IV 级升高（7.135、16.912 和 7.081 倍，均 P < 0.001）。所有 I-II-III-IV 级 SLC2A1 的 mRNA 水平均降低[0.424-、0.093-、0.234（均 P < 0.001）和 0.141 倍（均 P < 0.005）]。所有 I-II-III-IV 级 EGFR 的 mRNA 水平均升高[1.388、5.452（均 P < 0.017）、4.624-和 4.137 倍]。I-II-III 级 PTEN 的 mRNA 水平升高[1.802-、1.702-和 1.5 倍]，IV 级降低（0.176 倍）。所有 I-II-III-IV 级 CD276 的 mRNA 水平均升高[1.8-、5.756-（均 P < 0.001）、10.303（均 P < 0.001）和 2.5 倍]。

结论

我们获得了与先前报道的 PDK1、EGFR、PTEN 和 CD276 基因表达水平相似的结果。相比之下，SLC2A1 的表达明显下调，这与其他肿瘤研究报道一致。这些发现可能是由于脑肿瘤组织的独特性质所致。此外，在包括胶质母细胞瘤和脑膜瘤在内的 IV 级脑肿瘤中，PTEN 基因表达降低。尽管分析的研究组大小有限，但基因表达结果显示了在癌症分期过程中基因行为的相似性。