Yang Y, Shao N, Luo G, Li L, Nilsson-Ehle P, Xu N
Department of Neurosurgery, Third Affiliated Hospital of Suzhou University, Changzhou, China.
Scand J Clin Lab Invest. 2006;66(6):469-75. doi: 10.1080/00365510600763285.
To investigate the relationship between PTEN gene expression and differentiation of glioma.
The quantitative real-time reverse transcription-polymerase chain reaction (RT-PCR) method was applied to detect PTEN mRNA levels in glioma tissues. Tumor-adjacent normal tissues and benign brain tumors were used as controls. Relative PTEN mRNA levels were determined as the ratio of PTEN and GAPDH, which were correlated with the clinical-pathological results.
PTEN mRNA levels were significantly lower in the glioma tissues than in the benign brain tumors and tumor-adjacent normal tissues, whereas there were no statistical differences between benign brain tumor and the tumor-adjacent normal tissues. According to the pathological examinations, PTEN mRNA levels were higher in the high differential glioma than the low differential glioma.
PTEN gene expression was suppressed in the glioma, which is related to the clinical-pathological results. It is suggested that determination of PTEN mRNA levels by RT-PCR could be a novel marker of disease classification.
探讨PTEN基因表达与胶质瘤分化之间的关系。
应用定量实时逆转录-聚合酶链反应（RT-PCR）方法检测胶质瘤组织中PTEN mRNA水平。以肿瘤旁正常组织和良性脑肿瘤作为对照。将相对PTEN mRNA水平确定为PTEN与GAPDH的比值，并将其与临床病理结果相关联。
胶质瘤组织中PTEN mRNA水平显著低于良性脑肿瘤和肿瘤旁正常组织，而良性脑肿瘤与肿瘤旁正常组织之间无统计学差异。根据病理检查，高分化胶质瘤中的PTEN mRNA水平高于低分化胶质瘤。
胶质瘤中PTEN基因表达受到抑制，这与临床病理结果相关。提示通过RT-PCR测定PTEN mRNA水平可能是一种疾病分类的新标志物。
