Leininger Wes R, Peng Zhuoyu, Zhang Bo
Department of Chemistry, University of Washington, Seattle, Washington 98195, United States.
Chem Biomed Imaging. 2023 Mar 23;1(4):380-386. doi: 10.1021/cbmi.3c00020. eCollection 2023 Jul 24.
Here we report the use of a Langmuir isotherm model to analyze and better understand the dynamic adsorption and desorption behavior of single fluorophore molecules at the surface of a hydrogen nanobubble supported on an indium tin oxide (ITO) electrode. Three rhodamine dyes, rhodamine 110 (R110, positively charged), rhodamine 6G (R6G, positively charged), and sulforhodamine G (SRG, negatively charged) were chosen for this study. The use of the Langmuir isotherm model allows us to determine the equilibrium constant and the rate constants for the adsorption and desorption processes. Of the three fluorophores used in this study, SRG was found to have the greatest equilibrium constant. No significant potential dependence was observed on the adsorption characteristics, which suggests the nanobubble size, geometry, and surface properties are relatively constant within the range of potentials used in this study. Our results suggest that the use of the Langmuir isotherm model is a valid and useful means for probing and better understanding the unique adsorption behavior of fluorophores at surface-supported nanobubbles.
在此，我们报告使用朗缪尔等温线模型来分析并更好地理解单个荧光团分子在氧化铟锡（ITO）电极支撑的氢纳米气泡表面的动态吸附和解吸行为。本研究选择了三种罗丹明染料，罗丹明110（R110，带正电荷）、罗丹明6G（R6G，带正电荷）和磺基罗丹明G（SRG，带负电荷）。使用朗缪尔等温线模型使我们能够确定吸附和解吸过程的平衡常数和速率常数。在本研究使用的三种荧光团中，发现SRG具有最大的平衡常数。未观察到吸附特性对电位有明显依赖性，这表明在本研究使用的电位范围内，纳米气泡的尺寸、几何形状和表面性质相对恒定。我们的结果表明，使用朗缪尔等温线模型是探测和更好地理解荧光团在表面支撑的纳米气泡上独特吸附行为的有效且有用的方法。
