Nosip是肝癌细胞中的一个潜在治疗靶点。

Nosip is a potential therapeutic target in hepatocellular carcinoma cells.

作者信息

Gao Junjie, Yang Dandan, Huang Zheng, Pan Xueshan, Cao Ruoxue, Lian Chaoqun, Ma Jia, Li Yuyun, Wang Zhiwei, Xia Jun

机构信息

Bengbu Medical College Key Laboratory of Cancer Research and Clinical Laboratory Diagnosis, School of Laboratory Medicine, Bengbu Medical College, Bengbu 233030, Anhui, China.

Department of Clinical Laboratory, the Second Affiliated Hospital of Wannan Medical College, Wuhu 241001, Anhui, China.

出版信息

iScience. 2023 Jul 10;26(8):107353. doi: 10.1016/j.isci.2023.107353. eCollection 2023 Aug 18.

DOI:10.1016/j.isci.2023.107353

PMID:37529099

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10387614/

Abstract

Nitric oxide synthase-interacting protein (Nosip) interacts with nitric oxide synthase (NOS) and regulates NO synthesis and release, which participates in various critical physiological and pathological processes. However, the role of Nosip in hepatocellular carcinoma (HCC) is unclear. In this study, Nosip expression was found to be elevated in HCC tissues and cells. Nosip siRNA transfection inhibited the proliferation and motility of HCC cells and promoted apoptosis. In contrast, overexpression of Nosip promoted proliferation and migration and invasion, and inhibited apoptosis of HCC cells. As a natural compound, quercetin exerted the effect of inhibiting the proliferation and motility of HCC cells, and this anticancer activity probably via repressing the expression of Nosip. Our results suggest that Nosip could act as an oncogene in the progression of HCC and that quercetin may be a potential natural compound for treating HCC by inhibiting the expression of Nosip.

摘要

一氧化氮合酶相互作用蛋白（Nosip）与一氧化氮合酶（NOS）相互作用，调节NO的合成与释放，参与各种关键的生理和病理过程。然而，Nosip在肝细胞癌（HCC）中的作用尚不清楚。在本研究中，发现Nosip在HCC组织和细胞中表达升高。Nosip siRNA转染抑制了HCC细胞的增殖和运动能力，并促进了细胞凋亡。相反，Nosip的过表达促进了HCC细胞的增殖、迁移和侵袭，并抑制了细胞凋亡。作为一种天然化合物，槲皮素发挥了抑制HCC细胞增殖和运动能力的作用，这种抗癌活性可能是通过抑制Nosip的表达来实现的。我们的结果表明，Nosip可能在HCC进展中作为一种癌基因发挥作用，并且槲皮素可能是一种通过抑制Nosip表达来治疗HCC的潜在天然化合物。