Wang Bao-Zhu, Wang Meng-Meng, Li Yan, Shao Mei-Hua, Zhang Dan, Yan Shi-Qi, Ma Xiang, Ma Yi-Tong
Department of Cardiology of the First Affiliated Hospital, Xinjiang Medical University, Urumqi, P.R. China.
Department of Intensive Care Unit of the Fifth Affiliated Hospital, Xinjiang Medical University, Urumqi, P.R. China.
Turk J Biol. 2023 May 4;47(3):170-185. doi: 10.55730/1300-0152.2653. eCollection 2023.
Recent clinical developments in tissue bioengineering have applications in acute cardiac ischemia and infarction and include the use of stem cells that combine injectable scaffold material. This study aimed to evaluate the effects of adipose-derived stem cells (ADSCs) that combine the Matrigel scaffold on cardiac morphology/functions. The autologous ADSCs myocardial infarction (MI) model was induced by the permanent ligation method of the left anterior descending coronary artery (LAD). MI-operated rats were randomly divided into PBS group, Matrigel group, PBS plus ADSCs group (PBS+ADSCs), and Matrigel plus ADSCs group (Matrigel+ADSCs). Matrigel was used as an injectable scaffold. Rats with a 1-week-old myocardial infarction were injected with 2 × 10 labeled ADSCs in the border area of the ischemic heart. Heart function was determined by echocardiography. The hemodynamics, cardiac structure, and graft characteristics were evaluated. The ADSCs were successfully isolated and identified, demonstrating a good proliferative status and cell retention in the Matrigel. ADSCs+Matrigel exhibited the most improved heart functions (LVESD, LVEDD, LVFS, LVEF) compared to those of other groups (p < 0.05). ADSCs+Matrigel significantly reduced infarct size compared to other groups (p < 0.05). Cotransplantation of ADSCs and Matrigel showed the best effect on maintaining the thickness of the ventricular wall compared to the other groups (p < 0.05). Engrafted ADSCs played a role in the formation of the neovasculature in myocardial infarction. ADSCs+Matrigel triggered the greatest enhancement in arteriole density than other groups (p < 0.05). Cotransplanting with ADSCs and Matrigel showed significantly higher levels of cardiac troponin T (cTnT), NK2-transcription factor related locus-5 (Nkx2.5), von Willebrand factor (vWF) than the other groups (p < 0.05). In conclusion, this study demonstrated that cotransplanting ADSCs with Matrigel resulted in improved cardiac morphology and cardiac function in the rat model of myocardial infarction.
组织生物工程学领域最近的临床进展在急性心脏缺血和梗死方面有应用，包括使用结合可注射支架材料的干细胞。本研究旨在评估结合基质胶支架的脂肪来源干细胞（ADSCs）对心脏形态/功能的影响。通过左冠状动脉前降支（LAD）永久性结扎法诱导建立自体ADSCs心肌梗死（MI）模型。将接受MI手术的大鼠随机分为PBS组、基质胶组、PBS+ADSCs组和基质胶+ADSCs组（Matrigel+ADSCs）。基质胶用作可注射支架。对心肌梗死1周龄的大鼠在缺血心脏边缘区域注射2×10个标记的ADSCs。通过超声心动图测定心脏功能。评估血流动力学、心脏结构和移植特征。ADSCs成功分离并鉴定，显示出良好的增殖状态以及在基质胶中的细胞留存。与其他组相比，ADSCs+基质胶组的心脏功能（左室收缩末期内径、左室舒张末期内径、左室短轴缩短率、左室射血分数）改善最为明显（p<0.05）。与其他组相比，ADSCs+基质胶组显著减小了梗死面积（p<0.05）。与其他组相比，ADSCs和基质胶共移植对维持心室壁厚度的效果最佳（p<0.05）。植入的ADSCs在心肌梗死的新生血管形成中发挥作用。ADSCs+基质胶组比其他组引发的小动脉密度增加最为显著（p<0.05）。与其他组相比，ADSCs和基质胶共移植组的心肌肌钙蛋白T（cTnT）、NK2转录因子相关位点-5（Nkx2.5）、血管性血友病因子（vWF）水平显著更高（p<0.05）。总之，本研究表明在心肌梗死大鼠模型中，ADSCs与基质胶共移植可改善心脏形态和心脏功能。
