GDF10 作为一种来源于皮下脂肪组织的脂肪细胞因子，与肥胖有关。

GDF10 is related to obesity as an adipokine derived from subcutaneous adipose tissue.

机构信息

Department of Internal Medicine, Chungnam National University College of Medicine, Daejeon, Republic of Korea.

Research Center for Endocrine and Metabolic Disease, Chungnam National University College of Medicine, Daejeon, Republic of Korea.

出版信息

Front Endocrinol (Lausanne). 2023 Jul 14;14:1159515. doi: 10.3389/fendo.2023.1159515. eCollection 2023.

DOI:10.3389/fendo.2023.1159515

PMID:37529611

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10390302/

Abstract

INTRODUCTION

Adipokines are proteins that are secreted by the adipose tissue. Although they are associated with obesity-related metabolic disorders, most studies have focused on adipokines expressed by visceral adipose tissue (VAT). This study aimed to identify the adipokine potentially derived from subcutaneous adipose tissue (SAT) and its clinical significance.

METHODS

Samples of SAT and VAT were obtained from six adult male patients who underwent laparoscopic surgery for benign gall bladder disease. Differentially expressed genes were analyzed by subjecting the samples to RNA sequencing. The serum concentration of selected proteins according to body mass index (BMI) was analyzed in 58 individuals.

RESULTS

showed significantly higher expression in the SAT, as per RNA sequencing (fold change = 5.8, adjusted value = 0.009). Genes related to insulin response, glucose homeostasis, lipid homeostasis, and fatty acid metabolism were suppressed when expression was high in SAT, as per genotype-tissue expression data. The serum GDF10 concentration was higher in participants with BMI ≥ 25 kg/m (n = 35, 2674 ± 441 pg/mL) than in those with BMI < 25 kg/m (n = 23, 2339 ± 639 pg/mL; = 0.022). There was a positive correlation between BMI and serum GDF10 concentration (r = 0.308, = 0.019).

CONCLUSIONS

expression was higher in SAT than in VAT. Serum GDF10 concentration was high in patients with obesity. Therefore, GDF10 could be a SAT-derived protein related to obesity.

摘要

简介

脂肪因子是由脂肪组织分泌的蛋白质。尽管它们与肥胖相关的代谢紊乱有关，但大多数研究都集中在内脏脂肪组织（VAT）表达的脂肪因子上。本研究旨在确定潜在来源于皮下脂肪组织（SAT）的脂肪因子及其临床意义。

方法

从六名因良性胆囊疾病接受腹腔镜手术的成年男性患者中获取 SAT 和 VAT 样本。通过对样本进行 RNA 测序分析差异表达基因。根据体重指数（BMI）分析了 58 名个体中选定蛋白质的血清浓度。

结果

根据 RNA 测序，在 SAT 中表达明显更高（倍数变化=5.8，调整后的值=0.009）。根据基因-组织表达数据，当 SAT 中表达较高时，与胰岛素反应、葡萄糖稳态、脂质稳态和脂肪酸代谢相关的基因受到抑制。GDF10 血清浓度在 BMI≥25kg/m（n=35，2674±441pg/mL）的参与者中高于 BMI<25kg/m（n=23，2339±639pg/mL；=0.022）。BMI 与血清 GDF10 浓度之间呈正相关（r=0.308，=0.019）。