El Baiomy Mohamed A, Akl Tamer, ElMenshawy Nadia, El-Sebaie Ahmed H, Morkos Hanna, El-Ashwah Shaimaa, El-Sabbagh Amr M, Wahba Yahya, El-Ghonemy Mohamed
Department of Internal Medicine, Medical Oncology Unit, Mansoura University Oncology Center, Mansoura, Egypt.
Clinical Pathology Department, Hematology Unit, Mansoura University, Mansoura, Egypt.
Indian J Cancer. 2023 Apr-Jun;60(2):217-223. doi: 10.4103/ijc.IJC_102_21.
Interleukin-10 (IL-10) and tumor necrosis factor-alpha (TNF-α) genes contribute to oncogenesis. We evaluated the influence of the IL-10 (G1082A) and TNF-α (G308A) polymorphisms on the prognosis and outcomes of Egyptian patients with acute lymphoblastic leukemia (ALL).
We investigated 64 children and 76 adults with ALL, between 2016 and 2019, for the IL-10 (G1082A) and TNF-α (G308A) polymorphisms using allele-specific polymerase chain reaction and polymerase chain reaction-restriction fragment length polymorphism. Survival analyses were performed using the Kaplan-Meier estimator and the log-rank test.
In children with ALL, the A allele of TNF-α and IL-10 polymorphisms was associated with older age (P = 0.04 and 0.03), more extramedullary disease (P = 0.02 and 0.001), positive breakpoint cluster region-Abelson (BCR-ABL) rearrangement (p190; P = 0.04 and 0.001), and more relapse (P = 0.002). The IL-10 GG genotype was associated with higher overall survival in children (P = 0.026). Adults carrying the TNF-α A allele showed more extramedullary disease (P = 0.009) and relapse (P = 0.003). We also found a higher frequency of IL-10 A allele in adults with older age (P = 0.03), lower hemoglobin level (P = 0.04), positive BCR-ABL rearrangement (P = 0.001), more extramedullary disease (P = 0.001), more relapse (P = 0.002), and a longer time for the first complete remission (P = 0.003).
A possible association exists between the A allele of IL-10 and TNF-α polymorphisms and poor prognosis in Egyptian patients with ALL, while the IL-10 GG genotype may be associated with better survival in children with ALL.
白细胞介素-10（IL-10）和肿瘤坏死因子-α（TNF-α）基因参与肿瘤发生。我们评估了IL-10（G1082A）和TNF-α（G308A）基因多态性对埃及急性淋巴细胞白血病（ALL）患者预后和结局的影响。
在2016年至2019年期间，我们使用等位基因特异性聚合酶链反应和聚合酶链反应-限制性片段长度多态性方法，对64名儿童和76名成人ALL患者进行了IL-10（G1082A）和TNF-α（G308A）基因多态性检测。使用Kaplan-Meier估计器和对数秩检验进行生存分析。
在儿童ALL患者中，TNF-α和IL-10基因多态性的A等位基因与年龄较大（P = 0.04和0.03）、更多髓外疾病（P = 0.02和0.001）、阳性断裂点簇区域-阿贝尔森（BCR-ABL）重排（p190；P = 0.04和0.001）以及更多复发（P = 0.002）相关。IL-10 GG基因型与儿童较高的总生存率相关（P = 0.026）。携带TNF-α A等位基因的成人表现出更多髓外疾病（P = 0.009）和复发（P =
0.003）。我们还发现，年龄较大的成人ALL患者中IL-10 A等位基因频率较高（P = 0.03），血红蛋白水平较低（P = 0.04），BCR-ABL重排阳性（P = 0.001），髓外疾病更多（P = 0.001），复发更多（P = 0.002），首次完全缓解时间更长（P = 0.003）。
埃及ALL患者中，IL-10和TNF-α基因多态性的A等位基因与不良预后之间可能存在关联，而IL-10 GG基因型可能与儿童ALL患者更好的生存率相关。
