Kizilbash Nadeem, Suhail Nida, Alzahrani A Khuzaim, Basha W Jamith, Soliman Mohamed
Department of Medical Laboratory Technology, Faculty of Applied Medical Sciences, Northern Border University, Arar, Saudi Arabia.
Department of Microbiology, Faculty of Medicine, Northern Border University, Arar, Saudi Arabia.
Indian J Pathol Microbiol. 2023 Jul-Sep;66(3):556-559. doi: 10.4103/ijpm.ijpm_1262_21.
The most accepted definition of regulatory T cells (T) relies on the expression of several biomarkers, including CD4, CD25, and transcription factor, Foxp3. The T maintain tolerance to self-antigens and prevent autoimmune diseases.
The purpose of this study was to determine the difference in natural T levels in Entamoeba histolytica, Schistosoma mansoni, Giardia lamblia, Enterobius vermicularis, and Hymenolepis nana infected patients.
Fifty-one pediatric subjects (29 males and 22 females) were recruited from a tertiary care hospital, and were divided into infected and non-infected (control) groups. The mean age of the subjects was 8.7 years.
Blood samples were collected from infected and non-infected groups, and change in the level of T in these subjects was investigated by flow cytometry.
The statistical analysis of data was performed by SPSS software. Quantitative data used in this study included mean and standard deviation. Data from the two groups were compared by the Student's t-test. The age of the patient and infection status were used for multivariate logistic regression analysis. Odds ratios (ORs) were estimated within a 95% confidence interval, and a P value of <0.05 was considered significant.
The levels of natural regulatory T cells, indicated by the biomarkers, CD4, CD25, and Foxp3, increase significantly in patients infected by Entamoeba histolytica, Schistosoma mansoni, Giardia lamblia, Enterobius vermicularis, and Hymenolepis nana as compared to controls. They also increase in cases of mixed infection as compared to infection by a single parasite.
调节性T细胞（Treg）最被广泛接受的定义依赖于几种生物标志物的表达，包括CD4、CD25和转录因子Foxp3。Treg维持对自身抗原的耐受性并预防自身免疫性疾病。
本研究的目的是确定感染溶组织内阿米巴、曼氏血吸虫、蓝氏贾第鞭毛虫、蠕形住肠线虫和微小膜壳绦虫的患者中自然Treg水平的差异。
从一家三级护理医院招募了51名儿科受试者（29名男性和22名女性），并将其分为感染组和非感染（对照）组。受试者的平均年龄为8.7岁。
从感染组和非感染组采集血样，并通过流式细胞术研究这些受试者中Treg水平的变化。
数据的统计分析通过SPSS软件进行。本研究中使用的定量数据包括均值和标准差。两组数据通过学生t检验进行比较。患者的年龄和感染状态用于多因素逻辑回归分析。比值比（OR）在95%置信区间内估计，P值<0.05被认为具有统计学意义。
与对照组相比，感染溶组织内阿米巴、曼氏血吸虫、蓝氏贾第鞭毛虫、蠕形住肠线虫和微小膜壳绦虫的患者中，由生物标志物CD4、CD25和Foxp3所指示的自然调节性T细胞水平显著升高。与单一寄生虫感染相比，混合感染病例中的Treg水平也升高。
