School of Food Science and Pharmaceutical Engineering, Nanjing Normal University, Nanjing, P. R. China.
J Food Sci. 2023 Sep;88(9):3807-3819. doi: 10.1111/1750-3841.16700. Epub 2023 Aug 2.
The aim of the present study was to prepare a new antimicrobial Pickering emulsion of which the star anise essential oil was added to the oil phase, and to investigate the effect of stabilization by bio-based active nanoparticles consisting of zein and pectin loaded with thymol. First, the thymol-loaded zein/pectin composite nanoparticles (ZTNPs) were fabricated as uniformly distributed spherical nanoparticles with an average diameter of 200 nm through antisolvent precipitation. Second, the effects of nanoparticles' concentration, oil phase ratio, and storage time on the stability of emulsions were explored according to particle size potential, interfacial tension, rheology, and micromorphology. Finally, the antibacterial results showed that Pickering emulsion inhibited Escherichia coli and Staphylococcus aureus compared to the control group by nearly 7 log colony-forming unit/g at 36 h, which was twice as much as the inhibition by thymol or star anise essential oils and ZTNPs. Therefore, the proposed Pickering emulsion with star anise essential oil could be used as a green and safe plant-derived antimicrobial agent in the food industry.
本研究旨在制备一种新的抗菌 Pickering 乳液，其中添加了八角茴香油到油相中，并研究由负载百里香的玉米醇溶蛋白和果胶组成的生物基活性纳米粒子稳定的效果。首先，通过反溶剂沉淀法制备了百里香负载的玉米醇溶蛋白/果胶复合纳米粒子（ZTNPs），其为具有平均直径 200nm 的均匀分布的球形纳米粒子。其次，根据粒径电位、界面张力、流变学和微观形态，探讨了纳米粒子浓度、油相比例和储存时间对乳液稳定性的影响。最后，抑菌结果表明，与对照组相比，Pickering 乳液在 36 小时内对大肠杆菌和金黄色葡萄球菌的抑制率接近 7 个对数菌落形成单位/g，是百里香或八角茴香油和 ZTNPs 的抑菌效果的两倍。因此，所提出的含有八角茴香油的 Pickering 乳液可以用作食品工业中一种绿色、安全的植物源抗菌剂。
