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全反式维甲酸诱导分化因子（ATRAID）的功能特性

Functional characterization of all-trans retinoic acid-induced differentiation factor (ATRAID).

作者信息

Mehrasa Roya, Cristea Ileana, Bredrup Cecilie, Rødahl Eyvind, Bruland Ove

机构信息

Department of Clinical Medicine, University of Bergen, Norway.

Department of Medical Genetics, Haukeland University Hospital, Bergen, Norway.

出版信息

FEBS Open Bio. 2023 Oct;13(10):1874-1886. doi: 10.1002/2211-5463.13685. Epub 2023 Sep 7.

DOI:10.1002/2211-5463.13685
PMID:37530719
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10549228/
Abstract

All-trans retinoic acid-induced differentiation (ATRAID) factor was first identified in HL60 cells. Several mRNA isoforms exist, but the respective proteins have not been fully characterized. In transfected cells expressing Myc-Flag-tagged ATRAID Isoform (Iso) A, B, and C, Iso C was found to be expressed at high levels, Iso A was found to be expressed at low levels due to rapid degradation, and the predicted protein expressed from Iso B was not detected. Iso C was present mainly in an N-glycosylated form. In subcellular fractionation experiments, Iso C localized to the membranous and nuclear fractions, while immunofluorescence analysis revealed that Iso C is located close to the plasma membrane, mainly in cytoplasmic vesicles and in the Golgi area. We confirm that Iso C colocalizes to some extent with endosomal/lysosomal markers LAMP1 and LAMP2. Furthermore, we show that ATRAID co-localizes with RAB11, a GTPase associated with recycling endosomes and implicated in regulating vesicular trafficking.

摘要

全反式维甲酸诱导分化（ATRAID）因子最初是在HL60细胞中被鉴定出来的。存在几种mRNA异构体，但各自的蛋白质尚未得到充分表征。在表达Myc-Flag标记的ATRAID异构体（Iso）A、B和C的转染细胞中，发现Iso C高水平表达，Iso A由于快速降解而低水平表达，并且未检测到从Iso B表达的预测蛋白质。Iso C主要以N-糖基化形式存在。在亚细胞分级分离实验中，Iso C定位于膜和核部分，而免疫荧光分析显示Iso C位于靠近质膜的位置，主要存在于细胞质囊泡和高尔基体区域。我们证实Iso C在一定程度上与内体/溶酶体标记物LAMP1和LAMP2共定位。此外，我们表明ATRAID与RAB11共定位，RAB11是一种与回收内体相关的GTP酶，参与调节囊泡运输。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ef0/10549228/26d97a40d742/FEB4-13-1874-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ef0/10549228/d209de0cf9fe/FEB4-13-1874-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ef0/10549228/f80b9be27e15/FEB4-13-1874-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ef0/10549228/ad9b45868810/FEB4-13-1874-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ef0/10549228/98049ec094d6/FEB4-13-1874-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ef0/10549228/26d97a40d742/FEB4-13-1874-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ef0/10549228/d209de0cf9fe/FEB4-13-1874-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ef0/10549228/f80b9be27e15/FEB4-13-1874-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ef0/10549228/ad9b45868810/FEB4-13-1874-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ef0/10549228/98049ec094d6/FEB4-13-1874-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ef0/10549228/26d97a40d742/FEB4-13-1874-g006.jpg

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