Tecnológico Nacional de México, Instituto Tecnológico El Llano Aguascalientes, Km. 18 Carretera Aguascalientes-San Luis Potosí, 20330, El Llano, Aguascalientes, México.
Centro Nacional de Investigación Disciplinaria en Salud Animal e Inocuidad, INIFAP, Carretera Cuernavaca-Cuautla 8534, Col. Progreso, 62550, Jiutepec, Morelos, México.
Acta Parasitol. 2023 Sep;68(3):683-689. doi: 10.1007/s11686-023-00703-z. Epub 2023 Aug 2.
This work aimed to study the seroprevalence of natural infection by T. gondii in different population segments of dogs from Aguascalientes, Mexico as well as its possible association with some risk factors.
The study included 210 clinically healthy dogs from three population segments: rural, stray and pet. A blood serum sample was collected on a single occasion and processed by indirect immunofluorescence considering a dilution of 1:16 as a positive reaction; the association between the presence of antibodies and the potential risk factors was estimated with logistic regression.
The overall prevalence of anti-T. gondii antibodies in the studied population was 59% (123/210; CI 95% 51-65) being different between population segments, in rural dogs 74% (59/80; CI 95% 62-82), in stray dogs 60% (48/80; CI 95% 48-70) and in pet dogs 32% (16/50; CI 95% 19-46), the titers of the positive sera were in a range of 1:16-1:128. In rural dogs the seroprevalence according to the municipality was in a range of 55% to 100% finding positive dogs in all the studied municipalities. The zootechnical function of guarding the property in rural dogs was identified as a risk factor (OR 2.4), while in pet dogs it was living with cats (OR 7) as well as sharing the drinking water container (OR 4); in stray dogs it was not possible to identify any risk factor.
This study documents the wide presence of anti-T. gondii antibodies in the population groups examined, this being more important in rural dogs.
本研究旨在调查墨西哥阿瓜斯卡连特斯州不同犬种群体中自然感染弓形虫的血清流行率，并分析其与某些危险因素的相关性。
本研究共纳入 210 只临床健康犬，分为三个群体：农村犬、流浪犬和宠物犬。采集单次血清样本，采用间接免疫荧光法检测，稀释度为 1:16 时判为阳性反应。采用逻辑回归分析抗体存在与潜在危险因素之间的相关性。
研究人群的抗弓形虫抗体总阳性率为 59%（123/210；95%CI 95%为 51-65），不同群体间存在差异，农村犬阳性率为 74%（59/80；95%CI 95%为 62-82），流浪犬阳性率为 60%（48/80；95%CI 95%为 48-70），宠物犬阳性率为 32%（16/50；95%CI 95%为 19-46）。阳性血清的滴度范围为 1:16-1:128。根据所在城市，农村犬的血清阳性率在 55%-100%之间，在所研究的所有城市均发现了阳性犬。农村犬的看家功能被认为是一个危险因素（OR 2.4），而宠物犬与猫一起生活（OR 7）以及共用饮水容器（OR 4）也是危险因素；流浪犬则无法确定任何危险因素。
本研究记录了在所研究的犬种群体中广泛存在抗弓形虫抗体，农村犬中更为常见。
