King Fahd Medical Research Center, King Abdulaziz University, Jeddah 21589, Saudi Arabia; Yousef Abdul Latif Jameel Scientific Chair of Prophetic Medicine Application, Faculty of Medicine, King Abdulaziz University, Jeddah 21589, Saudi Arabia.
Department of Community Medicine, Faculty of Medicine, King Abdulaziz University, Jeddah 21589, Saudi Arabia.
Biomed Pharmacother. 2023 Sep;165:115236. doi: 10.1016/j.biopha.2023.115236. Epub 2023 Jul 31.
trans-Anethole a valuable compound derived from star anise widely used by ethnic tribals to manage numerous human diseases. In this study antiproliferative activities of trans-Anethole towards human liver cancer (HepG2), cervical cancer (HeLa) and breast cancer (MCF-7) cells were explored. trans-Anethole showed free radical scavenging potential as assessed by DNA nicking assay. trans-Anethole exhibited strong antiproliferative potential towards HepG2 cells compared to other cell lines. trans-Anethole strongly induced apoptosis in HepG2 cells by significantly upregulating the protein expressions of p53, Caspase-3 and Caspase-9 were assessed by western blotting analysis which highlighted apoptosis-inducing capacity of trans-Anethole against HepG2 cells. Rt-qPCR analysis revealed that trans- Anethole upregulated p53, caspase - 3 and - 9 in comparison to untreated HepG2 cancer cells. Moreover, trans-Anethole provoked the generation of ROS and disruption of MMP. Our research suggests that trans-Anethole may have a significant anticancer therapeutic potential for treating liver cancer.
反式茴香脑是一种从八角茴香中提取的有价值的化合物，被少数民族广泛用于治疗多种人类疾病。在这项研究中，研究了反式茴香脑对人肝癌（HepG2）、宫颈癌（HeLa）和乳腺癌（MCF-7）细胞的增殖抑制活性。通过 DNA 切口试验评估，反式茴香脑表现出自由基清除潜力。与其他细胞系相比，反式茴香脑对 HepG2 细胞表现出更强的增殖抑制潜力。通过 Western blot 分析评估，反式茴香脑显著上调 p53、Caspase-3 和 Caspase-9 的蛋白表达，从而强烈诱导 HepG2 细胞凋亡，这突出了反式茴香脑对 HepG2 细胞的凋亡诱导能力。Rt-qPCR 分析显示，与未处理的 HepG2 癌细胞相比，反式茴香脑上调了 p53、caspase-3 和 caspase-9。此外，反式茴香脑引发了 ROS 的产生和 MMP 的破坏。我们的研究表明，反式茴香脑可能具有治疗肝癌的显著抗癌治疗潜力。
