Department of Biomedical Engineering, College of Engineering, Virginia Commonwealth University, Richmond, VA, United States.
Department of Microbiology and Immunology, School of Medicine, Virginia Commonwealth University, Richmond, VA, United States.
Acta Biomater. 2023 Oct 1;169:605-624. doi: 10.1016/j.actbio.2023.07.052. Epub 2023 Aug 1.
Physiochemical cues like topography and wettability can impact the inflammatory response and tissue integration after biomaterial implantation. T cells are essential for immunomodulation of innate immune cells and play an important role in the host response to biomaterial implantation. This study aimed to understand how CD4 and CD8 T cell subsets, members of the αβ T cell family, polarize in response to smooth, rough, or rough-hydrophilic titanium (Ti) implants and whether their presence modulates immune cell crosstalk and mesenchymal stem cell (MSC) recruitment following biomaterial implantation. Post-implantation in mice, we found that CD4 and CD8 T cell subsets polarized differentially in response to modified Ti surfaces. Additionally, mice lacking αβ T cells had significantly more pro-inflammatory macrophages, fewer anti-inflammatory macrophages, and reduced MSC recruitment in response to modified Ti post-implantation than αβ T cell -competent mice. Our results demonstrate that T cell activation plays a significant role during the inflammatory response to implanted biomaterials, contributing to macrophage polarization and MSC recruitment and proliferation, and the absence of αβ T cells compromises new bone formation at the implantation site. STATEMENT OF SIGNIFICANCE: T cells are essential for immunomodulation and play an important role in the host response to biomaterial implantation. Our results demonstrate that T cells actively participate during the inflammatory response to implanted biomaterials, controlling macrophage phenotype and recruitment of MSCs to the implantation site.
理化线索，如形貌和润湿性，可影响生物材料植入后的炎症反应和组织整合。T 细胞对于固有免疫细胞的免疫调节至关重要，并在宿主对生物材料植入的反应中发挥重要作用。本研究旨在了解 CD4 和 CD8 T 细胞亚群（αβ T 细胞家族的成员）如何对光滑、粗糙或粗糙亲水钛（Ti）植入物作出反应而发生极化，以及它们的存在是否会调节免疫细胞串扰和间充质干细胞（MSC）在生物材料植入后的募集。在小鼠植入后，我们发现 CD4 和 CD8 T 细胞亚群对修饰 Ti 表面的反应存在差异极化。此外，与具有 αβ T 细胞能力的小鼠相比，缺乏 αβ T 细胞的小鼠在修饰 Ti 植入后表现出更多的促炎巨噬细胞、更少的抗炎巨噬细胞和减少的 MSC 募集。我们的结果表明，T 细胞激活在植入生物材料后的炎症反应中发挥重要作用，促进巨噬细胞极化和 MSC 的募集和增殖，而缺乏 αβ T 细胞会损害植入部位的新骨形成。
T 细胞对于免疫调节至关重要，并在宿主对生物材料植入的反应中发挥重要作用。我们的结果表明，T 细胞在植入生物材料后的炎症反应中积极参与，控制巨噬细胞表型和 MSC 向植入部位的募集。
