Department of Forensic Medicine and Toxicology, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt.
Department of Physiology, Faculty of Veterinary Medicine, Zagazig University, 44511, Zagazig, Sharkia, Egypt.
Food Chem Toxicol. 2023 Sep;179:113977. doi: 10.1016/j.fct.2023.113977. Epub 2023 Jul 31.
This study delves into the intricate exploration of potential toxic effects resulting from subchronic exposure to fenpropathrin (FNP) on the reproductive system of male SD rats. Adding to the novelty, our study undertakes a pioneering comparison of the effects of curcumin (CUR) and curcumin-encapsulated chitosan nanoparticles (CS.CUR.NPs) on these toxic effects. The study involved a cohort of sixty male SD rats (six groups): vehicle control, CUR, Cs.CUR.NPs, FNP, and two combination groups (FNP with CUR or Cs.CUR.NPs). The synthesized Cs.CUR.NPs nanoparticles underwent meticulous characterization using Fourier Infrared spectroscopy (FT-IR) and transmission electron microscopy (TEM). The findings revealed that FNP caused oxidative stress, sperm abnormalities, reduced motility and sperm count FNP decreased serum LH, FSH, 17-β estradiol, and testosterone levels. FNP downregulated the mRNA expression of the spermatogenesis and steroidogenesis-related genes, While, downregulated hypothalamic KISS-1 and KISS-1r expression. Histopathological alterations were assessed and scored. Surprisingly, the treatment with CUR and Cs.CUR.NPs exhibited remarkable restorative effects on semen quality, sex hormone levels, antioxidant capacity, and mRNA expression of the targeted genes. Notably, Cs.CUR.NPs displayed superior properties when compared to CUR. Nevertheless, further research is imperative to evaluate their efficacy across various bodily tissues.
本研究深入探讨了氯氟氰菊酯（FNP）亚慢性暴露对雄性 SD 大鼠生殖系统潜在毒性作用。此外，本研究还开创性地比较了姜黄素（CUR）和姜黄素包被壳聚糖纳米粒子（CS.CUR.NPs）对这些毒性作用的影响。该研究涉及 60 只雄性 SD 大鼠（6 组）：对照组、CUR 组、CS.CUR.NPs 组、FNP 组和两个联合组（FNP 与 CUR 或 CS.CUR.NPs）。合成的 CS.CUR.NPs 纳米粒子经过傅里叶变换红外光谱（FT-IR）和透射电子显微镜（TEM）的详细表征。研究结果表明，FNP 引起氧化应激、精子异常、运动能力和精子计数降低，FNP 降低了血清 LH、FSH、17-β 雌二醇和睾酮水平。FNP 下调了与精子发生和类固醇生成相关的基因的 mRNA 表达，而下调了下丘脑 KISS-1 和 KISS-1r 的表达。评估和评分了组织病理学改变。令人惊讶的是，CUR 和 CS.CUR.NPs 的治疗对精液质量、性激素水平、抗氧化能力和靶基因的 mRNA 表达表现出显著的恢复作用。值得注意的是，与 CUR 相比，CS.CUR.NPs 具有更好的性能。然而，仍需要进一步研究来评估它们在各种身体组织中的功效。
