National Centre for Nanoscience and Nanotechnology, University of Madras, Chennai 600 025, India; Dermscientist Laboratory Pvt., 11/D2, Jawaharlal Street, Usman Road, Chennai, India.
National Centre for Nanoscience and Nanotechnology, University of Madras, Chennai 600 025, India.
Int J Biol Macromol. 2023 Oct 1;250:126001. doi: 10.1016/j.ijbiomac.2023.126001. Epub 2023 Jul 31.
Magnetized iron oxide nanoparticles are ideal materials for biological and biomedical applications due to their biocompatibility, super paramagnetic behavior, surface capability, and chemical stability. This research article is narrating the overview of methodologies of preparation, functionalization, characterization and applications of FeO nanoparticles. Super paramagnetic nanoparticles are studied for their hyperthermia properties. The proposed mechanism behind the hyperthermia was damaging the proteins responsible for DNA repair thereby, directly accelerating the DNA damages on cancer cells by increasing the temperature in the vicinity of the cancer cells. In this study, super paramagnetic iron oxide (FeO) nanoparticles (SPIONs) and anti-cancer drug, 5-fluorouracil, functionalized with N-Hydroxysuccinimide organic molecules. A specific absorption rate at 351 nm can be achieved using UV analysis. The magnetic FeO nanoparticles had a cubic crystalline structure. FE-SEM(field emission scanning Electron microscopy) with EDAX(energy dispersive X-ray analysis) analysis shows that the size of the SPION was about 30-100 nm range and the percentage of chemical compositions was higher in the order of Fe, O, C. for particle size analysis, the SPION were positively charged derived at +9.9 mV and its conductivity is measured at 0.826 mS/cm. In-vitro anti-cancerous activity analysis in Hep-G2 cells (liver cancer cells) shows that the 5-fluorouracil functionalized SPIONs have higher inhibition rate than the bare FeO nanoparticles. The FeO nanoparticles were studied for their hyperthermic abilities at two different frequencies such as 3.05 × 10 kAms and 4.58 × 10 kAms.The bare FeO at low magnetic field, 10 mg was required to raise the temperature above 42°- 45 °C and at high magnetic field, 6 mg was enough to raise the same temperature. The 5-fluorouracil functionalized FeO shows that at low magnetic field, 6 mg is required to raise the hyperthermia temperature and at high magnetic field, 3 mg is required to raise the temperature above 42°- 45 °C. the rate of heating and the temperature achieved with time can be tuned with concentrations as well as magnetic component present in the FeO nanoparticles. Beyond this concentration, the rate of cell death was observed to increase. The saturation and low residual magnetization were revealed by the magnetization analysis, making them well suited for clinical applications.
磁性氧化铁纳米粒子由于其生物相容性、超顺磁性、表面性能和化学稳定性，是生物和生物医学应用的理想材料。本文综述了 FeO 纳米粒子的制备、功能化、表征和应用方法。超顺磁纳米粒子因其热疗特性而受到研究。热疗背后的机制被认为是破坏负责 DNA 修复的蛋白质，从而通过增加癌细胞附近的温度，直接加速癌细胞的 DNA 损伤。在这项研究中，超顺磁氧化铁（FeO）纳米粒子（SPION）和抗癌药物 5-氟尿嘧啶（5-FU）与 N-羟基琥珀酰亚胺有机分子功能化。使用紫外分析可以达到 351nm 的特定吸收率。磁性 FeO 纳米粒子具有立方晶结构。FE-SEM（场发射扫描电子显微镜）与 EDAX（能量色散 X 射线分析）分析表明，SPION 的尺寸约为 30-100nm 范围，化学组成的百分比按 Fe、O、C 的顺序更高。对于粒径分析，SPION 带正电荷，衍生出+9.9mV，其电导率为 0.826mS/cm。在 Hep-G2 细胞（肝癌细胞）中的体外抗癌活性分析表明，5-氟尿嘧啶功能化的 SPION 比裸 FeO 纳米粒子具有更高的抑制率。在两种不同频率（3.05×10kAms 和 4.58×10kAms）下研究了 FeO 纳米粒子的热疗能力。在低磁场下，裸 FeO 需要 10mg 才能将温度升高到 42°-45°C，而在高磁场下，6mg 就足以将温度升高到相同的温度。5-氟尿嘧啶功能化的 FeO 表明，在低磁场下，需要 6mg 才能升高热疗温度，而在高磁场下，需要 3mg 才能将温度升高到 42°-45°C。可以通过浓度以及 FeO 纳米粒子中存在的磁性成分来调整加热速率和随时间达到的温度。超过这个浓度，观察到细胞死亡速率增加。通过磁化分析揭示了饱和和低剩余磁化强度，使它们非常适合临床应用。
Cancer Biother Radiopharm. 2017-12
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