莨菪烷和澳洲茄碱在铜绿假单胞菌中生物膜刺激活性的研究。
Biofilm stimulating activity of solanidine and Solasodine in Pseudomonas aeruginosa.
机构信息
Department of Microbiology, College of Sciences, Agriculture and Modern Technology, Shiraz Branch, Islamic Azad University, Shiraz, Iran.
Department of Medical Microbiology (Bacteriology and Virology), Afzalipour Faculty of Medicine, Kerman University of Medical Sciences, Kerman, Iran.
出版信息
BMC Microbiol. 2023 Aug 2;23(1):208. doi: 10.1186/s12866-023-02957-z.
BACKGROUND
Biofilm formation has reported as an important virulence associated properties of Pseudomonas aeruginosa that is regulated by quorum-sensing associated genes. Biofilm and quorum-sensing interfering properties of steroidal alkaloids, Solanidine and Solasodine were investigated in the present study.
RESULTS
Biofilm formation capacity and relative expression level of five studied genes(lasI, lasR, rhlI, rhlR and algD) were significantly increased dose-dependently after treatment with sub-inhibitory concentrations (32 and 512 µg/ml) of the both Solanidine and Solasodine. Biofilm formation capacity was more stimulated in weak biofilm formers(9 iaolates) in comparison to the strong biofilm producers(11 isolates). The lasI gene was the most induced QS-associated gene among five investigated genes.
CONCLUSION
Biofilm inducing properties of the plants alkaloids and probably medicines derived from them has to be considered for revision of therapeutic guidelines. Investigating the biofilm stimulating properties of corticosteroids and other medicines that comes from plant alkaloids also strongly proposed.
背景
生物膜的形成被认为是铜绿假单胞菌的一个重要毒力相关特性，它受到群体感应相关基因的调节。本研究调查了甾体生物碱薯蓣皂素和茄啶的生物膜和群体感应干扰特性。
结果
用亚抑制浓度（32 和 512μg/ml）处理后，两种薯蓣皂素和茄啶均可剂量依赖性地显著增加生物膜形成能力和五种研究基因（lasI、lasR、rhlI、rhlR 和 algD）的相对表达水平。与强生物膜生产者（11 株）相比，弱生物膜形成者（9 株）的生物膜形成能力受到更强的刺激。lasI 基因是五个研究基因中最被诱导的群体感应相关基因。
结论
植物生物碱的生物膜诱导特性及其可能从中衍生的药物，必须在修订治疗指南时加以考虑。强烈建议研究皮质类固醇和其他源自植物生物碱的药物的生物膜刺激特性。
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