A New Distinctive Darwin Wasp Represents the First Record of the Species-group (Hymenoptera: Ichneumonidae: Ophioninae) from Japan and the Far East, with an Analysis of DNA Barcode-based Species Delimitation in .

A new Darwin wasp species, Shimizu sp. nov. (Hymenoptera: Ichneumonidae: Ophioninae), is described using the integrated morphological and molecular species delimitation approaches. Our results indicate that the new species is closely related to European Gravenhorst, 1829 of the species-group but can be distinguished using morphological characters, such as entirely black body colour with some light-yellow marks and not inclined epicnemial carina in lateral view. This record of the new species represents the first record of species-group from Japan and the Far East. Phylogenetic analysis indicate that the species-group is weakly recovered as monophyletic and sister to The analysis also indicated that two clades within the species-group ( and ) diverged significantly. This suggests that the species-group, as well as the two included clades, could potentially be treated as separate species-groups or genera. The present study supports previous integrative taxonomic and phylogenetic studies of and represents a first fundamental step for studies focused on the challenging Japanese and Far Eastern .