College of Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, 300193, People's Republic of China.
Key Laboratory of Basic and Application Research of Beiyao (Heilongjiang University of Chinese Medicine), Ministry of Education, Harbin, 150040, People's Republic of China.
Drug Des Devel Ther. 2023 Jul 28;17:2223-2237. doi: 10.2147/DDDT.S409064. eCollection 2023.
Black plaster is one of the classic dosage forms of traditional Chinese medicine for external use and has been widely utilized since the Tang and Song Dynasties. In this paper, we take Goupi Gao as the research object and discuss the scientific characteristics of the black plaster dosage form. Goupi Gao ointment is a plaster for external use of traditional Chinese medicine.
Methods for the morphological and quantitative characterization of black plaster's microstructure, based on FESEM-IPP (Field Emission Scanning Electron Microscope IPP Image Processing) technology, were established. According to the actual operating temperature of Goupi Gao, three temperatures were selected: 28°C, 35°C, and 45°C. A UPLC analysis method was applied to the cinnamaldehyde and eugenol in Goupi Gao, and the release behavior of Goupi Gao from three samples at three temperatures was investigated using the paddle over disk method. Preparation of rabbit model of knee osteoarthritis of cold blood stasis type by cold stimulation combined with drug induction.
In terms of morphology, Goupi Gao and the blank black plaster matrix both formed a double continuous phase system with a thicker vegetable oil phase and crossed "branched" soap crystal fibers. Based on the IPP image quantification parameters, the pore area (A) was highly positively correlated with temperature. After the 28 °C treatment, A = (216.8±59.5) μm; after the 35 °C treatment, A = (259.7±52.8) μm; after the 45 °C treatment, A = (408.0±57.7) μm, and there were no significant differences in other pore parameters.
The black plaster matrix's unique structure makes it highly applicable in numerous medications; it exhibits slow-release and performs well in extreme temperatures, with good adhesion and peeling properties.
黑膏药是中医传统外用剂型之一，自唐宋以来一直广泛应用。本研究以狗皮膏为研究对象，探讨黑膏药剂型的科学特点。狗皮膏是一种中药外用膏药。
基于 FESEM-IPP（场发射扫描电子显微镜 IPP 图像处理）技术，建立了黑膏药微观结构形态和定量特征的方法。根据狗皮膏的实际操作温度，选择了三个温度：28°C、35°C 和 45°C。采用 UPLC 分析方法对狗皮膏中的肉桂醛和丁香酚进行分析，并采用桨碟法考察了三个温度下三个样品的狗皮膏释放行为。采用冷刺激结合药物诱导法制备家兔寒凝血瘀型膝骨关节炎模型。
从形态上看，狗皮膏和空白黑膏药基质均形成了较厚的植物油相和交叉“分支”的皂晶纤维的双连续相体系。基于 IPP 图像量化参数，孔面积（A）与温度呈高度正相关。28°C 处理后，A=（216.8±59.5）μm；35°C 处理后，A=（259.7±52.8）μm；45°C 处理后，A=（408.0±57.7）μm，其他孔参数无显著差异。
黑膏药基质独特的结构使其在多种药物中具有广泛的应用价值；它具有缓释作用，在极端温度下表现良好，具有良好的附着力和剥离性能。
