She Weiyi, Wang Hao, Linardi Darwin, Chik Sin Yu, Lan Yi, Chen Feng, Cheng Aifang, Qian Pei-Yuan
Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou), Nansha, Guangdong, China.
Department of Ocean Science and Hong Kong Brach of Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou), Hong Kong University of Science and Technology, Hong Kong, China.
iScience. 2023 May 28;26(7):106981. doi: 10.1016/j.isci.2023.106981. eCollection 2023 Jul 21.
Marine biofouling causes huge economic losses to the marine industry every year. Albofungin is a potential antifoulant showing strong anti-macrofouling activities against larval settlement of major fouling organisms. In the present study, directed RNA-seq and proteomic analyses were used to investigate changes in the transcriptome and proteome of a major fouling barnacle cyprids in response to albofungin treatment. Results showed that albofungin treatment remarkably upregulated the metabolism of xenobiotics by the cytochrome P450 pathway to discharge the compound and downregulated energy metabolic processes. Intriguingly, immunostaining and whole-mount in situ hybridization (WISH) revealed the spatial expression patterns of selected differentially expressed genes (glutathione S-transferase [GST], nitric oxide synthase [NOS], and calmodulin [CaM]) distributed in the thorax and antennule of . Our study provides new insights into the mechanism of albofungin in interrupting the larval settlement of and suggests its potential application as an antifouling agent in marine environments.
海洋生物污损每年给海洋产业造成巨大经济损失。阿博霉素是一种潜在的防污剂，对主要污损生物的幼虫附着表现出强烈的抗大型污损活性。在本研究中，使用定向RNA测序和蛋白质组学分析来研究主要污损藤壶无节幼虫在阿博霉素处理后的转录组和蛋白质组变化。结果表明，阿博霉素处理显著上调了细胞色素P450途径对外源化合物的代谢以排出该化合物，并下调了能量代谢过程。有趣的是，免疫染色和全组织原位杂交（WISH）揭示了选定的差异表达基因（谷胱甘肽S-转移酶[GST]、一氧化氮合酶[NOS]和钙调蛋白[CaM]）在藤壶胸部和触角中的空间表达模式。我们的研究为阿博霉素阻断藤壶幼虫附着的机制提供了新的见解，并表明其在海洋环境中作为防污剂的潜在应用。
