感染重塑红细胞及其细胞外囊泡的人类微小RNA谱。

infection reshapes the human microRNA profiles of red blood cells and their extracellular vesicles.

作者信息

Wu Yifan, Leyk Stephanie, Torabi Hanifeh, Höhn Katharina, Honecker Barbara, Tauler Maria Del Pilar Martinez, Cadar Dániel, Jacobs Thomas, Bruchhaus Iris, Metwally Nahla Galal

机构信息

Research Group Host Parasite Interaction, Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany.

Research Group Protozoa Immunology, Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany.

出版信息

iScience. 2023 Jun 15;26(7):107119. doi: 10.1016/j.isci.2023.107119. eCollection 2023 Jul 21.

DOI:10.1016/j.isci.2023.107119

PMID:37534175

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10391920/

Abstract

, a human malaria parasite, develops in red blood cells (RBCs), which represent approximately 70% of all human blood cells. Additionally, RBC-derived extracellular vesicles (RBC-EVs) represent 7.3% of the total EV population. The roles of microRNAs (miRNAs) in the consequences of infection are unclear. Here, we analyzed the miRNA profiles of non-infected human RBCs (niRBCs), ring-infected RBCs (riRBCs), and trophozoite-infected RBCs (trRBCs), as well as those of EVs secreted from these cells. Hsa-miR-451a was the most abundant miRNA in all RBC and RBC-EV populations, but its expression level was not affected by infection. Overall, the miRNA profiles of RBCs and their EVs were altered significantly after infection. Most of the differentially expressed miRNAs were shared between RBCs and their EVs. A target prediction analysis of the miRNAs revealed the possible identity of the genes targeted by these miRNAs (CXCL10, OAS1, IL7, and CCL5) involved in immunomodulation.

摘要

疟原虫，一种人类疟原虫，在红细胞（RBCs）中发育，红细胞约占人类所有血细胞的70%。此外，红细胞衍生的细胞外囊泡（RBC-EVs）占总细胞外囊泡群体的7.3%。微小RNA（miRNAs）在感染后果中的作用尚不清楚。在这里，我们分析了未感染的人类红细胞（niRBCs）、环状感染的红细胞（riRBCs）和滋养体感染的红细胞（trRBCs）的miRNA谱，以及这些细胞分泌的细胞外囊泡的miRNA谱。Hsa-miR-451a是所有红细胞和红细胞细胞外囊泡群体中最丰富的miRNA，但其表达水平不受感染影响。总体而言，感染后红细胞及其细胞外囊泡的miRNA谱发生了显著变化。大多数差异表达的miRNA在红细胞及其细胞外囊泡之间是共享的。对这些miRNA的靶标预测分析揭示了这些miRNA（CXCL10、OAS1、IL7和CCL5）靶向的可能参与免疫调节的基因身份。