Wan Weisong, Wang Le, Liu Yufei, Hu Yuchang
The First College of Clinical Medical Science, China Three Gorges University, Yichang, China.
Institute of Pathology, China Three Gorges University, Yichang, China.
Front Oncol. 2023 Jul 17;13:1229871. doi: 10.3389/fonc.2023.1229871. eCollection 2023.
The objective of the study was to compare the consistency of various staining methods, including H&E, Methylene Blue, Warthin-Starry (W-S), Immunohistochemistry (IHC) and Quantum dots immunohistochemistry (QDs-IHC), in detecting (HP) in cases of mild, moderate and severe chronic gastritis.
Biopsy samples were obtained from 225 patients with chronic gastritis at the Department of Pathology, Yichang Central People's Hospital between January 2019 and October 2019. The presence of HP was detected using H&E, Methylene Blue, W-S, IHC, and QDs-IHC.
The positive rates for HP detection using H&E, Methylene Blue, W-S, IHC, and QDs-IHC were 42.22%, 51.11%, 53.78%, 59.11%, and 58.67%, respectively. In cases of mild chronic gastritis, the consistency of test results between H&E, Methylene Blue, W-S, and QDs-IHC with IHC were Kappa=0.196, =0.033, Kappa=0.706, P<0.001, Kappa=0.717, <0.001, and Kappa=0.968, <0.001, respectively. Similarly, in cases of moderate chronic gastritis, Kappa values between H&E, Methylene Blue, W-S, and QDs-IHC with IHC were 0.356, <0.001, 0.655, <0.001, 0.741, <0.001, and 0.946, <0.001, respectively. For cases of severe chronic gastritis, the Kappa values between the staining methods and IHC were 0.271, =0.037, 0.421, =0.002, 0.621, <0.001, and 1, < 0.001, respectively.
The study showed that the positivity rate of IHC was significantly higher than that of H&E, Methylene Blue, and W-S in detecting HP infection in chronic gastritis cases. In terms of consistency with IHC, QDs-IHC was the most reliable staining method across all severity grades, while the agreement between H&E and IHC was poor, and that between Methylene Blue and W-S with IHC was average. Pathology departments may choose the most appropriate staining method based on their specific needs, considering the staining time, contrast, and cost of each method.
本研究的目的是比较多种染色方法，包括苏木精-伊红染色（H&E）、亚甲蓝染色、沃辛-斯塔里染色（W-S）、免疫组织化学染色（IHC）和量子点免疫组织化学染色（QDs-IHC），在轻度、中度和重度慢性胃炎病例中检测幽门螺杆菌（HP）的一致性。
2019年1月至2019年10月期间，从宜昌市中心人民医院病理科的225例慢性胃炎患者中获取活检样本。使用H&E、亚甲蓝、W-S、IHC和QDs-IHC检测HP的存在情况。
使用H&E、亚甲蓝、W-S、IHC和QDs-IHC检测HP的阳性率分别为42.22%、51.11%、53.78%、59.11%和58.67%。在轻度慢性胃炎病例中，H&E、亚甲蓝、W-S和QDs-IHC与IHC检测结果的一致性Kappa值分别为0.196，P<0.001；0.033，P<0.001；0.706，P<0.001；0.717，P<0.001；0.968，P<0.001。同样，在中度慢性胃炎病例中，H&E、亚甲蓝、W-S和QDs-IHC与IHC的Kappa值分别为0.356，P<0.001；0.655，P<0.001；0.741，P<0.001；0.946，P<0.001。对于重度慢性胃炎病例，各染色方法与IHC的Kappa值分别为0.271，P=0.037；0.421，P=0.002；0.621，P<0.001；1，P<0.001。
研究表明，在慢性胃炎病例中检测HP感染时，IHC的阳性率显著高于H&E、亚甲蓝和W-S。就与IHC的一致性而言，QDs-IHC是所有严重程度分级中最可靠的染色方法，而H&E与IHC的一致性较差，亚甲蓝和W-S与IHC的一致性一般。病理科可根据各自的具体需求，考虑每种方法的染色时间、对比度和成本，选择最合适的染色方法。
