Cohen L F, Sayeeduddin M, Phillips C, Shahab I
Department of Pathology, Baylor College of Medicine and Ben Taub General Hospital, Houston, Texas 77030, USA.
Mod Pathol. 1997 Nov;10(11):1160-3.
Helicobacter pylori (HP) is prevalent in the general population and is associated with chronic active gastritis, peptic ulcers, gastric carcinoma, and lymphoma. Different methods to diagnose HP colonization include the urea breath test, serologic analysis, and gastric biopsy. Many different staining methods, including silver and Giemsa-based stains, have been used to demonstrate these organisms in gastric biopsy specimens. The tissue morphologic features, however, are often obscured, thus mandating evaluation of both hematoxylin and eosin (H&E) and special stains. This study evaluates the ability of a new staining method that allows simultaneous identification of HP and visualization of tissue morphologic features. We examined formalin-fixed, paraffin-embedded archival tissues from 184 consecutive gastric biopsy specimens using our new staining method, and we compared our results with those previously obtained from H&E and Warthin-Starry (WS) stains. Our new stain uses periodic acid-Schiff, Coleman's Feulgen solution, Mayer's hematoxylin, and methylene blue. Our method is technically simpler than the WS stain and can be completed in approximately 9 minutes. We found HP organisms in 76 (41%) of 184 biopsy specimens by H&E and/or WS staining. Our new staining method identified HP in 83 specimens (45%). The organisms stained bright blue and were easily visualized (compared with the H&E-stained specimens) because of the contrasting magenta mucin. Tissue morphologic features consisted of well-defined cells with dark blue nuclei and pale cytoplasm. Intestinal metaplasia was highlighted magenta Our new staining method revealed chronic active gastritis in 71 cases (39%), intestinal metaplasia in 18 cases (10%), and lymphoid aggregates in 35 cases (19%). These findings were similar to the results obtained with H&E-stained sections. We conclude that our new staining method is inexpensive, quick, and easy to perform and interpret. It has a sensitivity comparable to that of conventional staining methods and simultaneously demonstrates both tissue morphologic features and the presence of HP.
幽门螺杆菌（HP）在普通人群中普遍存在，与慢性活动性胃炎、消化性溃疡、胃癌和淋巴瘤有关。诊断HP定植的不同方法包括尿素呼气试验、血清学分析和胃活检。许多不同的染色方法，包括基于银和吉姆萨的染色，已被用于在胃活检标本中显示这些微生物。然而，组织形态学特征常常模糊不清，因此需要同时评估苏木精和伊红（H&E）染色以及特殊染色。本研究评估了一种新的染色方法的能力，该方法能够同时鉴定HP并可视化组织形态学特征。我们使用新的染色方法检查了184例连续胃活检标本的福尔马林固定、石蜡包埋存档组织，并将我们的结果与之前用H&E和沃辛-斯塔里（WS）染色获得的结果进行了比较。我们的新染色剂使用高碘酸-希夫试剂、科尔曼氏福尔根溶液、梅耶氏苏木精和亚甲蓝。我们的方法在技术上比WS染色更简单，大约9分钟即可完成。通过H&E和/或WS染色，我们在184例活检标本中的76例（41%）中发现了HP微生物。我们的新染色方法在83个标本（45%）中鉴定出了HP。这些微生物染成亮蓝色，由于品红色粘蛋白形成对比，很容易可视化（与H&E染色的标本相比）。组织形态学特征包括细胞核深蓝色、细胞质淡染的界限分明的细胞。肠化生被染成品红色。我们的新染色方法显示71例（39%）有慢性活动性胃炎，18例（10%）有肠化生，35例（19%）有淋巴滤泡。这些发现与H&E染色切片的结果相似。我们得出结论，我们的新染色方法价格低廉、快速且易于操作和解读。它的敏感性与传统染色方法相当，同时能显示组织形态学特征和HP的存在。
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