Purification of phospholamban and characterization of its antibodies.
作者信息
Holtzhauer M, Sydow H, Grunow G, Will H
出版信息
Biomed Biochim Acta. 1986;45(6):719-25.
PMID:3753477
Abstract
Phospholamban was extracted from pig heart microsomal membranes with a methanol-chloroform (2:1) mixture. The proteolipid was further purified to electrophoretic homogeneity by chromatography in organic solvents and in SDS-containing medium. Purification was about 50-fold with respect to specific 32P-phospholamban radioactivity. Antisera were produced in rabbits against phospholamban. Electroblot analysis demonstrates specific binding of the antisera to purified phospholamban and to phospholamban in preparations of cardiac sarcoplasmic reticulum and sarcolemma.
摘要
相似文献
1
Purification of phospholamban and characterization of its antibodies.
Biomed Biochim Acta. 1986;45(6):719-25.
2
The putative role of phospholamban in the regulation of the heart muscle.受磷蛋白在心肌调节中的假定作用。
Biomed Biochim Acta. 1986;45(1-2):S233-6.
3
Purification and characterization of phospholamban from canine cardiac sarcoplasmic reticulum.
J Biol Chem. 1985 Jun 25;260(12):7721-30.
4
Purification and characterization of phospholamban from canine cardiac sarcoplasmic reticulum.
J Biol Chem. 1985 Mar 25;260(6):3708-15.
5
Purification of phospholamban from bovine cardiac muscle with organic solvents.用有机溶剂从牛心肌中纯化受磷蛋白。
Arch Biochem Biophys. 1989 Mar;269(2):639-45. doi: 10.1016/0003-9861(89)90149-5.
6
Rapid purification of phospholamban by monoclonal antibody immunoaffinity chromatography.
Biochem Cell Biol. 1987 Apr;65(4):302-9. doi: 10.1139/o87-039.
7
Isolation of cardiac membrane proteolipids by high pressure liquid chromatography. A comparison of reticular and sarcolemmal proteolipids, phospholamban and calciductin.用高压液相色谱法分离心肌膜蛋白脂质。网状和肌膜蛋白脂质、受磷蛋白和钙转运蛋白的比较。
Biochim Biophys Acta. 1983 Feb 9;728(1):83-91. doi: 10.1016/0005-2736(83)90439-x.
8
Differences in phosphorylation of isolated phospholamban in dependence on the preparation procedure.分离的受磷蛋白磷酸化的差异取决于制备程序。
Biomed Biochim Acta. 1987;46(8-9):S465-7.
9
Comparison of the kinetic effects of phospholamban phosphorylation and anti-phospholamban monoclonal antibody on the calcium pump in purified cardiac sarcoplasmic reticulum membranes.磷蛋白磷酸化和抗磷蛋白单克隆抗体对纯化心肌肌浆网膜钙泵的动力学效应比较。
Biochemistry. 1997 Oct 21;36(42):12903-10. doi: 10.1021/bi971109v.
10
Phosphorylation of phospholamban by cAMP-dependent protein kinase enhances interactions between Ca-ATPase polypeptide chains in cardiac sarcoplasmic reticulum membranes.环磷酸腺苷依赖性蛋白激酶对受磷蛋白的磷酸化作用增强了心肌肌浆网膜中钙-ATP酶多肽链之间的相互作用。
Biochemistry. 1996 Sep 3;35(35):11247-59. doi: 10.1021/bi960864q.
Zotero 插件