Redmann K
Biomed Biochim Acta. 1986;45(6):749-54.
The transmembrane potential (TMP) of FL-cells (monolayer cultures) in Eagle's medium with ouabain concentrations from 10(-4) to 10(-11) M was measured by means of glass microelectrodes over 96 h. High ouabain concentrations caused total depolarization of the cell membrane with subsequent cell death after 24 to 48 h. A level of 10(-7) M ouabain was tolerated by the cells over 96 h, and the TMP was decreased about 50% vs. controls in ouabain-free medium. Ouabain at concentrations from 10(-11) to 10(-8) M increases TMP by 20-40%, which decayed after 24 to 48 h. The low glycoside concentrations are assumed to cause, if only part of the glycoside receptors (Na+ pumps) is blocked, an activation of the still active pumps (turnover) and enhanced formation of transport enzymes and their incorporation into the membrane (replacement) so that the overall result is a stimulation of the cell membrane transport systems.
在含有浓度为10⁻⁴至10⁻¹¹M哇巴因的伊格尔培养基中，采用玻璃微电极在96小时内测量了FL细胞（单层培养）的跨膜电位（TMP）。高浓度的哇巴因会导致细胞膜完全去极化，并在24至48小时后导致细胞死亡。细胞在96小时内可耐受10⁻⁷M的哇巴因水平，与不含哇巴因的培养基中的对照组相比，TMP降低了约50%。浓度为10⁻¹¹至10⁻⁸M的哇巴因可使TMP升高20 - 40%，这种升高在24至48小时后衰减。低浓度糖苷被认为，即使只有部分糖苷受体（钠泵）被阻断，仍会激活仍在起作用的泵（周转率），增强转运酶的形成及其并入膜中（替换），从而总体结果是刺激细胞膜转运系统。
