The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Edinburgh, Midlothian, United Kingdom.
Scotland's Rural College, Edinburgh, United Kingdom.
Bioinformatics. 2023 Aug 1;39(8). doi: 10.1093/bioinformatics/btad483.
Accurate gene prediction is essential for successful metagenome analysis. We present KOunt, a Snakemake pipeline, that precisely quantifies KEGG orthologue abundance.
KOunt is available on GitHub: https://github.com/WatsonLab/KOunt. The KOunt reference database is available on figshare: https://doi.org/10.6084/m9.figshare.21269715. Test data are available at https://doi.org/10.6084/m9.figshare.22250152 and version 1.2.0 of KOunt at https://doi.org/10.6084/m9.figshare.23607834.
准确的基因预测对于成功的宏基因组分析至关重要。我们提出了 KOunt,一个 Snakemake 管道,它可以精确地量化 KEGG 直系同源物的丰度。
KOunt 可在 GitHub 上获得:https://github.com/WatsonLab/KOunt。KOunt 参考数据库可在 figshare 上获得:https://doi.org/10.6084/m9.figshare.21269715。测试数据可在 https://doi.org/10.6084/m9.figshare.22250152 上获得,而 KOunt 的 1.2.0 版本可在 https://doi.org/10.6084/m9.figshare.23607834 上获得。