School of Bioengineering, Dalian University of Technology, Dalian 116024, China.
Institute of Environmental Systems Biology, College of Environmental Science and Engineering, Dalian Maritime University, Dalian 116026, China.
J Agric Food Chem. 2023 Aug 16;71(32):12264-12279. doi: 10.1021/acs.jafc.3c03412. Epub 2023 Aug 3.
Tomato is a globally important horticultural and economic crop, but its productivity is severely affected by various stresses. Plant small secretory peptides have been identified as crucial mediators in plant resistance. Here, we conducted a comparative transcriptome analysis and identified the gene from (), as an ortholog of encoding the precursor protein of PAMP-induced SSP 1. The expression level of is transcriptionally induced in tomato upon infection with (), the pathogen responsible for late blight. Overexpression of resulted in enhanced tomato resistance to . In addition, exogenous application of SpPIP1, whether through spraying or irrigation, improved tomato resistance by enhancing the transcript accumulations of pathogenesis-related proteins, as well as reactive oxygen species and the jasmonic acid (JA) levels. Integrated analysis of transcriptomics and metabolomics revealed the potential contributions of JA and phenylpropanoid biosynthesis to SpPIP1-induced tomato immunity. Additionally, SpPIP1 may strengthen tomato resistance to salt stress through the ABA signaling pathway. Overall, our findings demonstrate that SpPIP1 positively regulates tomato tolerance to and salt stress, making it a potential plant elicitor for crop protection in an environmentally friendly way.
番茄是一种全球重要的园艺作物和经济作物，但它的生产力受到各种胁迫的严重影响。植物小分泌肽已被确定为植物抗性的重要介质。在这里，我们进行了比较转录组分析，从番茄中鉴定出一个基因，作为编码 PAMP 诱导的 SSP1 前体蛋白的 的同源物。在感染引起晚疫病的病原菌 （）后，番茄中 的表达水平在转录水平上被诱导。过表达 导致番茄对 的抗性增强。此外，通过喷雾或灌溉外源应用 SpPIP1 提高了番茄对 的抗性，增强了病程相关蛋白、活性氧和茉莉酸 (JA) 水平的转录积累。转录组学和代谢组学的综合分析揭示了 JA 和苯丙烷生物合成对 SpPIP1 诱导的番茄免疫的潜在贡献。此外，SpPIP1 可能通过 ABA 信号通路增强番茄对盐胁迫的抗性。总的来说，我们的研究结果表明，SpPIP1 正向调节番茄对 和盐胁迫的耐受性，使其成为一种潜在的植物诱导剂，可用于以环保的方式保护作物。
