Altern Ther Health Med. 2023 Oct;29(7):444-446.
Extravillous trophoblasts (EVTs) cells have shown promise for their application in non-invasive prenatal diagnosis during the first trimester. The Trophoblast Retrieval and Isolation from the Cervix (TRIC) method allows for the isolation of homogeneous trophoblast cells from pregnant women as early as 5 weeks gestation.
This study aimed to explore the potential value of extravillous trophoblast cells collected from the cervix, enriched, and purified using the TRIC method for first-trimester prenatal diagnosis.
A prospective observational study was conducted, and we collected extravillous trophoblast cells from the cervixes of 100 pregnant women between 5-7 weeks gestation before an induced abortion. Subsequently, these cells underwent STR analysis and fluorescence in situ hybridization (FISH).
Out of the 100 cases, trophoblast cells were successfully collected from 96 cases. Among them, STR analysis revealed maternal cell contamination in 13 cases. Gender determination using FISH showed 44 male cases (including one case with 47, XY, +21) and 39 female cases (including one case with 47, XXX). The results of the FISH examination of these 83 cases were in concordance with those of the villi FISH examination.
The collection of fetal trophoblast cells from the cervix represents a feasible and non-invasive approach for first-trimester prenatal diagnosis. The TRIC method enables efficient enrichment and purification of trophoblast cells, which can be of significant benefit for subsequent diagnosis using the FISH method.
滋养层外细胞（EVTs）在早孕期的非侵入性产前诊断中具有应用前景。宫颈滋养层细胞回收和分离（TRIC）方法允许从妊娠 5 周的孕妇中分离出同质的滋养层细胞。
本研究旨在探讨使用 TRIC 方法从宫颈中分离、富集和纯化的绒毛外滋养层细胞在早孕期产前诊断中的潜在价值。
进行了一项前瞻性观察性研究，我们在 100 例妊娠 5-7 周的孕妇自愿行人工流产前，从宫颈采集绒毛外滋养层细胞。随后，对这些细胞进行 STR 分析和荧光原位杂交（FISH）。
在 100 例中，96 例成功采集到滋养层细胞。其中，STR 分析显示 13 例存在母细胞污染。FISH 性别鉴定显示 44 例男性（包括 1 例 47，XY，+21）和 39 例女性（包括 1 例 47，XXX）。83 例 FISH 检查结果与绒毛 FISH 检查结果一致。
从宫颈采集胎儿滋养层细胞是一种可行的、非侵入性的早孕期产前诊断方法。TRIC 方法能够有效地富集和纯化滋养层细胞，这对于后续使用 FISH 方法进行诊断具有重要意义。
