Heyuan Branch, Guangdong Provincial Laboratory of Lingnan Modern Agricultural Science and Technology & Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou 510642, PR China; Guangdong Engineering Research Center for Vector Vaccine of Animal Virus, Guangzhou 510642, PR China; South China Collaborative Innovation Center for Poultry Disease Control and Product Safety, Guangzhou 510642, PR China; Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangzhou 510642, Guangdong, PR China.
Henry Fok School of Biology and Agriculture, Shaoguan University, Shaoguan 510642, PR China.
Vet Microbiol. 2023 Sep;284:109821. doi: 10.1016/j.vetmic.2023.109821. Epub 2023 Jun 22.
While the presence of host cell proteins in virions and their role in viral life cycles have been demonstrated in various viruses, such characteristics have remained largely unknown in avian leukosis virus (ALV). To investigate whether this is the case in ALV, we purified high-integrity and high-purity virions from the avian leukosis virus subgroup J (ALV-J) and subjected them to proteome analysis using nano LC-MS/MS. This analysis identified 53 cellular proteins that are incorporated into mature ALV-J virions, and we verified the reliability of the packaged cellular proteins through subtilisin digestion and immunoblot analysis. Functional annotation revealed the potential functions of these proteins in the viral life cycle and tumorigenesis. Overall, our findings have important implications for understanding the interaction between ALV-J and its host, and provide new insights into the cellular requirements that define ALV-J infection.
虽然在各种病毒中已经证明了宿主细胞蛋白在病毒粒子中的存在及其在病毒生命周期中的作用，但在禽白血病病毒（ALV）中，这些特征在很大程度上仍然未知。为了研究这是否适用于 ALV，我们从禽白血病病毒亚群 J（ALV-J）中纯化了高完整性和高纯度的病毒粒子，并使用纳升 LC-MS/MS 对其进行了蛋白质组分析。该分析鉴定了 53 种整合到成熟 ALV-J 病毒粒子中的细胞蛋白，我们通过枯草杆菌蛋白酶消化和免疫印迹分析验证了包装的细胞蛋白的可靠性。功能注释揭示了这些蛋白质在病毒生命周期和肿瘤发生中的潜在功能。总的来说，我们的发现对于理解 ALV-J 与其宿主之间的相互作用具有重要意义，并为定义 ALV-J 感染的细胞需求提供了新的见解。
