Li Xiaofei, Wang Qi, Gao Yanni, Qi Xiaole, Wang Yongqiang, Gao Honglei, Gao Yulong, Wang Xiaomei
Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China.
Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China ; Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Disease and Zoonoses, Yangzhou 225009, China.
Biomed Res Int. 2015;2015:395307. doi: 10.1155/2015/395307. Epub 2015 Jan 8.
Avian leukosis virus subgroup J (ALV-J) is an avian oncogenic retrovirus that can induce various clinical tumors and has caused severe economic losses in China. To improve our understanding of the host cellular responses to virus infection and the pathogenesis of ALV-J infection, we applied isobaric tags for relative and absolute quantification (iTRAQ) labeling coupled with multidimensional liquid chromatography-tandem mass spectrometry to detect the protein changes in DF-1 cells infected and mock-infected with ALV-J. A total of 75 cellular proteins were significantly changed, including 33 upregulated proteins and 42 downregulated proteins. The reliability of iTRAQ-LC MS/MS was confirmed via real-time PCR. Most of these proteins were related to the physiological functions of metabolic processes, biosynthetic processes, responses to stimuli, protein binding, signal transduction, cell cytoskeleton, and so forth. We also found some proteins that play important roles in apoptosis and oncogenicity. The differentially expressed proteins identified may provide valuable information to elucidate the pathogenesis of virus infection and virus-host interactions.
禽白血病病毒J亚群（ALV-J）是一种禽致癌逆转录病毒，可诱发多种临床肿瘤，在中国已造成严重经济损失。为了增进我们对宿主细胞对病毒感染的反应以及ALV-J感染发病机制的理解，我们应用相对和绝对定量等压标签（iTRAQ）标记结合多维液相色谱-串联质谱法，来检测感染和未感染ALV-J的DF-1细胞中的蛋白质变化。共有75种细胞蛋白发生了显著变化，其中包括33种上调蛋白和42种下调蛋白。通过实时PCR证实了iTRAQ-LC MS/MS的可靠性。这些蛋白大多与代谢过程、生物合成过程、刺激反应、蛋白结合、信号转导、细胞细胞骨架等生理功能有关。我们还发现了一些在细胞凋亡和致癌性中起重要作用的蛋白。所鉴定出的差异表达蛋白可能为阐明病毒感染的发病机制和病毒-宿主相互作用提供有价值的信息。
