State Key Laboratory of Subtropical Silviculture, School of Forestry & Biotechnology, Zhejiang A&F University, Lin'an, Hangzhou, 311300, Zhejiang, PR China.
State Key Laboratory of Subtropical Silviculture, School of Forestry & Biotechnology, Zhejiang A&F University, Lin'an, Hangzhou, 311300, Zhejiang, PR China.
Plant Physiol Biochem. 2023 Sep;202:107913. doi: 10.1016/j.plaphy.2023.107913. Epub 2023 Jul 26.
Phoebe is a well-known timber tree species that contains abundant metabolites characterized by flavonoids that are widely used in the pharmaceutical industry. Nevertheless, temporospatial flavonoid metabolism variations substantially impact the Phoebe industry. Thus, a metabolomics analysis was carried out and identified 465 metabolites (102 flavonoids) in P. bournei, revealing distinct distribution patterns among five studied organs, and most of the flavonoids were dominant in the leaves. Furthermore, three kaempferol glycoside derivatives were significantly accumulated in the leaves and showed higher contents in young leaves than in mature leaves and differences between spring and autumn. For instance, greater accumulation of kaempferol-7-O-rhamnoside was detected in spring, whereas higher contents of kaempferol-3-O-arabinofuranoside and kaempferol-3-O-rhamnoside were found in autumn. Integrated metabolomics and transcriptomics identified 20 transcription factors (TFs) and 12 structural genes that participate in kaempferol derivative synthesis and elucidated a potential regulatory mechanism in P. bournei. Of the identified genes, PbMYB211 might contribute significantly to the kaempferol-3-O-rhamnoside content by regulating the target structural gene PbUGT139, as revealed by transient overexpression analysis. Overall, this study illuminated the temporospatial accumulation of flavonoids among different organs, seasons, and developmental stages in P. bournei and elucidated a potential regulatory pathway of kaempferol-3-O-rhamnoside. The results provide important insights into harvest techniques and a theoretical basis for the comprehensive utilization of P. bournei.
菲比是一种著名的木材树种，其富含以类黄酮为特征的代谢物，广泛应用于制药行业。然而，时空黄酮代谢变化对菲比产业有重大影响。因此，进行了代谢组学分析，在朴树中鉴定出 465 种代谢物（102 种类黄酮），揭示了五种研究器官中明显的分布模式，大部分类黄酮在叶片中占优势。此外，三种山柰酚糖苷衍生物在叶片中显著积累，且在嫩叶中的含量高于成熟叶和春秋两季之间的差异。例如，山柰酚-7-O-鼠李糖苷在春季的积累量较大，而山柰酚-3-O-阿拉伯呋喃糖苷和山柰酚-3-O-鼠李糖苷在秋季的含量较高。代谢组学和转录组学综合分析鉴定出 20 个转录因子（TFs）和 12 个结构基因参与山柰酚衍生物的合成，并阐明了朴树中潜在的调控机制。在所鉴定的基因中，PbMYB211 可能通过调节靶结构基因 PbUGT139 对山柰酚-3-O-鼠李糖苷的含量有显著贡献，这是通过瞬时过表达分析揭示的。总之，本研究阐明了朴树不同器官、季节和发育阶段黄酮类化合物的时空积累情况，并阐明了山柰酚-3-O-鼠李糖苷的潜在调控途径。研究结果为朴树的采收技术提供了重要的见解，并为朴树的综合利用提供了理论依据。
