Shao Yi, Hong Runqing, Li Boyao, Wang Ao, Chen Yuru, Wang Yingyu, Mo Fei, Liu Mingchun, Tian Chunlian
Key Laboratory of Livestock Infectious Diseases in Northeast China, Ministry of Education, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, No 120 Dongling Road Shenhe Dist., 110866 Shenyang, Liaoning Prov., People's Republic of China.
Key Laboratory of Livestock Infectious Diseases in Northeast China, Ministry of Education, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, No 120 Dongling Road Shenhe Dist., 110866 Shenyang, Liaoning Prov., People's Republic of China; Key Laboratory of Molecular Pharmacology and Drug Evaluation (Yantai University), Ministry of Education, Yantai University, 264005 Yantai, Shangdong Prov., People's Republic of China.
Fitoterapia. 2023 Oct;170:105630. doi: 10.1016/j.fitote.2023.105630. Epub 2023 Aug 1.
Artemisia anomala S. Moore exerts many pharmacological activities, including the removing of the blood stasis, relieving of the fever and analgesia, reducing the swelling and dampness. In this study, the extraction technology, chemical compositions and anti-inflammatory effect in vitro and mechanism of total flavonoids extract from Artemisia anomala S. Moore were studied. The optimal yield rate of total flavonoids extract was optimized by single factor experiments and response surface method, and the chemical constituents were analyzed by UPLC-QTOF-MS method; and the anti-inflammatory activity of the extract was evaluated with lipopolysaccharide induced RAW 264.7 cells. The highest extraction rate was 2.02% under these conditions of the concentration of ethanol 50%, the ultrasonic extraction time 30 min, and the ratio of solvent volume to material weight 20:1 (ml/g). In addition, the main components of total flavonoid extract were preliminarily identified and deduced based on mass spectrometry information and relevant literatures, and its stronger anti-inflammatory activity was demonstrated by reducing the phagocytosis, the content of nitric oxide and the level of related cytokines (tumor necrosis factor-α, interleukin-10, interleukin-6). Furthermore, it was further revealed that the anti-inflammatory effect of the extract was closely connected with the activation of TLR4-MyD88-NF-κB signalling pathway. This study indicated that the total flavonoids extract from Artemisia anomala S. Moore may be a better candidate anti-inflammatory natural medicine.
奇蒿发挥着多种药理活性，包括活血化瘀、清热止痛、消肿祛湿。本研究对奇蒿总黄酮提取物的提取工艺、化学成分、体外抗炎作用及机制进行了研究。通过单因素实验和响应面法优化了总黄酮提取物的最佳得率，采用超高效液相色谱-四极杆飞行时间质谱法分析了化学成分；并用脂多糖诱导的RAW 264.7细胞评价了提取物的抗炎活性。在乙醇浓度50%、超声提取时间30分钟、溶剂体积与物料重量比20:1（ml/g）的条件下，最高提取率为2.02%。此外，基于质谱信息和相关文献对总黄酮提取物的主要成分进行了初步鉴定和推导，并通过降低吞噬作用、一氧化氮含量和相关细胞因子（肿瘤坏死因子-α、白细胞介素-10、白细胞介素-6）水平证明了其较强的抗炎活性。此外，进一步揭示了提取物的抗炎作用与TLR4-MyD88-NF-κB信号通路的激活密切相关。本研究表明，奇蒿总黄酮提取物可能是一种较好的抗炎天然药物候选物。
