Department of Nutrition, Health Sciences Research Center, Faculty of Public Health, Mazandaran University of Medical Sciences, Sari, Iran.
Department of Physiotherapy, Faculty of Rehabilitation, Semnan University of Medical Sciences, Semnan, Iran.
J Chem Neuroanat. 2023 Oct;132:102322. doi: 10.1016/j.jchemneu.2023.102322. Epub 2023 Aug 1.
Identifying effective spinal cord injury (SCI) treatments remains a major challenge, and current approaches are still unable to effectively improve its. Currently, we investigated the combined effects of hyperbaric oxygen (HBO) along with coenzyme Q10 (CoQ10) in the recovery of SCI in rats.
Ninety female mature Sprague-Dawley rats were allocated into five equal groups, including; sham group, SCI group, HBO group (underwent SCI and received HBO), CoQ10 group (underwent SCI and received CoQ10), and HBO+CoQ10 group (underwent SCI and received HBO plus CoQ10). Tissue samples at the lesion site were obtained for evaluation of stereological, immunohistochemical, biochemical, molecular. Also, functional tests were performed to evaluate of behavioral properties.
We found that a significant increase in stereological parameters, biochemical factors (GSH, SOD and CAT), IL-10 gene expression and behavioral functions (BBB and EMG Latency) in the treatment groups, especially HBO+CoQ10 group, compared to SCI group. In addition, MDA levels, the density of apoptotic cells, as well as expression of inflammatory genes (TNF-α and IL-1β) were considerably reduced in the treatment groups, especially HBO+CoQ10 group, compared to SCI group.
We conclude that co-administration of HBO and HBO+CoQ10 has a synergistic neuroprotective effects in animals undergoing SCI.
鉴定有效的脊髓损伤 (SCI) 治疗方法仍然是一个主要挑战，目前的方法仍然无法有效地改善 SCI。目前，我们研究了高压氧 (HBO) 与辅酶 Q10 (CoQ10) 联合治疗大鼠 SCI 的效果。
90 只成熟雌性 Sprague-Dawley 大鼠被分为五组，包括假手术组、SCI 组、HBO 组（SCI 后接受 HBO）、CoQ10 组（SCI 后接受 CoQ10）和 HBO+CoQ10 组（SCI 后接受 HBO 加 CoQ10）。在损伤部位取组织样本进行体视学、免疫组织化学、生化、分子评价。还进行了功能测试以评估行为特性。
我们发现治疗组的体视学参数、生化因子（GSH、SOD 和 CAT）、IL-10 基因表达和行为功能（BBB 和 EMG 潜伏期）显著增加，尤其是 HBO+CoQ10 组，与 SCI 组相比。此外，治疗组 MDA 水平、凋亡细胞密度以及炎症基因（TNF-α 和 IL-1β）的表达明显降低，尤其是 HBO+CoQ10 组，与 SCI 组相比。
我们得出结论，HBO 与 CoQ10 联合给药对 SCI 动物具有协同的神经保护作用。
