分离的大鼠肝脏线粒体中荧光黄素蛋白的光谱特性
Spectral properties of fluorescent flavoproteins of isolated rat liver mitochondria.
作者信息
Kunz W S
出版信息
FEBS Lett. 1986 Jan 20;195(1-2):92-6. doi: 10.1016/0014-5793(86)80137-5.
PMID:3753688
Abstract
The different flavoproteins contributing to flavin fluorescence of isolated rat liver mitochondria have distinct excitation and emission spectra. The NAD-linked flavin component was identified as alpha-lipoamide dehydrogenase, while the non-NAD-linked component was found to be electron transfer flavoprotein. The differences in excitation and emission properties of the mitochondrial flavoproteins permit selective recording of their redox state changes in isolated mitochondria.
摘要
对分离出的大鼠肝脏线粒体黄素荧光有贡献的不同黄素蛋白具有不同的激发光谱和发射光谱。与NAD相关的黄素成分被鉴定为α-硫辛酸脱氢酶，而非NAD相关成分则被发现是电子传递黄素蛋白。线粒体黄素蛋白激发和发射特性的差异使得在分离的线粒体中能够选择性地记录它们的氧化还原状态变化。
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