Koke J R, Wylie W, Wills M
Cytobios. 1981;32(127-128):139-45.
The contribution of flavoprotein fluorescence to redox sensitive cellular autofluorescence was studied in single isolated adult rat heart cells. Fluorescence was measured quantitatively under conditions stimulating flavoprotein fluorescence in cells subjected to no inhibitors (sham), to cyanide, to 2,4-dinitrophenol, and to hypoxia. It was found that fluorescence apparently due to flavoproteins could be measured in single cells, and the fluorescence was sensitive to the redox state of the cell. Comparison with NADH fluorescence from single cells indicated that, while flavoprotein fluorescence was less intense, excitation of flavoproteins caused less bleaching of fluorescence when compared to the effect of excitation on NADH fluorescence.
在单个分离的成年大鼠心脏细胞中研究了黄素蛋白荧光对氧化还原敏感的细胞自发荧光的贡献。在无抑制剂（假手术）、氰化物、2,4-二硝基苯酚和缺氧条件下刺激细胞中黄素蛋白荧光的情况下，对荧光进行了定量测量。结果发现，在单细胞中可以测量到明显归因于黄素蛋白的荧光，并且该荧光对细胞的氧化还原状态敏感。与单细胞中的NADH荧光比较表明，虽然黄素蛋白荧光强度较低，但与激发对NADH荧光的影响相比，黄素蛋白的激发引起的荧光漂白较少。
