PASTEUR, Département de chimie, École normale supérieure, PSL University, Sorbonne Université, CNRS, Paris, France.
Institut Curie, Paris Sciences et Lettres (PSL) Research University, Centre National de la Recherche Scientifique (CNRS), Paris, France.
Nat Biotechnol. 2024 Jun;42(6):872-876. doi: 10.1038/s41587-023-01893-7. Epub 2023 Aug 3.
Here we present a method to reduce the photobleaching of fluorescent proteins and the associated phototoxicity. It exploits a photophysical process known as reverse intersystem crossing, which we induce by near-infrared co-illumination during fluorophore excitation. This dual illumination method reduces photobleaching effects 1.5-9.2-fold, can be easily implemented on commercial microscopes and is effective in eukaryotic and prokaryotic cells with a wide range of fluorescent proteins.
在这里，我们提出了一种减少荧光蛋白的光漂白和相关光毒性的方法。它利用了一种光物理过程，称为反向系间穿越，我们在荧光团激发时通过近红外共照明来诱导这种过程。这种双照明方法将光漂白效应降低 1.5-9.2 倍，可以很容易地在商业显微镜上实现，并且对具有广泛荧光蛋白的真核和原核细胞都有效。
