FAS Center for Systems Biology, Department of Molecular and Cellular Biology, School of Engineering and Applied Sciences, Harvard University, Cambridge, Massachusetts, USA.
Nat Methods. 2018 Jan;15(1):47-51. doi: 10.1038/nmeth.4509. Epub 2017 Nov 20.
The slow maturation time of fluorescent proteins (FPs) limits the temporal accuracy of measurements of rapid processes such as gene expression dynamics and effectively reduces fluorescence signal in growing cells. We used high-precision time-lapse microscopy to characterize the maturation kinetics of 50 FPs that span the visible spectrum at two different temperatures in Escherichia coli cells. We identified fast-maturing FPs from this set that yielded the highest signal-to-noise ratio and temporal resolution in individual growing cells.
荧光蛋白（FPs）的成熟时间缓慢，限制了对快速过程（如基因表达动态）的测量的时间精度，并且实际上降低了生长细胞中的荧光信号。我们使用高精度延时显微镜在大肠杆菌细胞中在两个不同温度下对跨越可见光谱的 50 个 FP 的成熟动力学进行了表征。我们从该组中鉴定出了快速成熟的 FP，它们在单个生长细胞中产生了最高的信噪比和时间分辨率。
