使用大幅面数字扫描仪自动读取DNA测序凝胶放射自显影片。

Elder J K, Green D K, Southern E M

Nucleic Acids Res. 1986 Jan 10;14(1):417-24. doi: 10.1093/nar/14.1.417.

We have developed a large format digital scanner for several applications in nucleic acid analysis. Here we describe the scanning of autoradiographs of DNA sequencing gels and a set of programs for reading the base sequence. The programs correct distortions in the gel, recognize bands by their characteristic shape and assign bases to bands by weighting band position and intensity. The sequence read in this way is as accurate as that read by an expert.

我们已经开发了一种大幅面数字扫描仪，用于核酸分析中的多种应用。在此，我们描述了对DNA测序凝胶放射自显影片的扫描以及一套用于读取碱基序列的程序。这些程序可校正凝胶中的变形，通过条带的特征形状识别条带，并通过权衡条带位置和强度为条带分配碱基。以这种方式读取的序列与专家读取的序列一样准确。