DNAGEL：一种计算机程序，用于使用配备图形输入板的小型计算机确定DNA片段大小。
DNAGEL: a computer program for determining DNA fragment sizes using a small computer equipped with a graphics tablet.
作者信息
Kieser T
出版信息
Nucleic Acids Res. 1984 Jan 11;12(1 Pt 2):679-88. doi: 10.1093/nar/12.1part2.679.
The program DNAGEL is used to determine the size of DNA fragments run on agarose or polyacrylamide gels. The positions of the bands are read from gel photographs by means of a digitizer. Standard curves are calculated by the method of Southern (1979). The bands, as they are measured, are reproduced on the screen so that erroneous input can be recognized and corrected immediately. Similarly the estimated fragment sizes are printed in a table in the same relative positions as the bands on the gel. This makes it especially easy to relate fragment sizes with the bands on the gel picture. As an additional function the calculated positions of bands can be displayed on the screen. The program DNAGEL is written in APPLESOFT BASIC, suitable for APPLE II computers with 48K memory connected to a monitor, printer and a HOUSTON graphics tablet.
程序DNAGEL用于确定在琼脂糖凝胶或聚丙烯酰胺凝胶上电泳的DNA片段大小。通过数字化仪从凝胶照片上读取条带位置。标准曲线采用Southern（1979年）的方法计算得出。测量的条带会在屏幕上重现，以便能立即识别并纠正错误输入。同样，估计的片段大小会以与凝胶上条带相同的相对位置打印在表格中。这使得将片段大小与凝胶图片上的条带关联起来变得格外容易。作为一项附加功能，计算出的条带位置可以显示在屏幕上。程序DNAGEL用APPLESOFT BASIC编写，适用于配备48K内存、连接到显示器、打印机和休斯顿图形输入板的苹果II型计算机。
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