Stocker T, Scott I, Šlapeta J
Sydney School of Veterinary Science, Faculty of Science, The University of Sydney, Sydney, New South Wales, 2006, Australia.
School of Veterinary Science, Massey University, Palmerston North, 4410, New Zealand.
Aust Vet J. 2023 Oct;101(10):373-376. doi: 10.1111/avj.13272. Epub 2023 Aug 3.
Hookworms (Ancylostomatidae) are well-known parasites in dogs due to their health impacts and zoonotic potential. While faecal analysis is the traditional method for detection, improvements in husbandry and deworming have decreased their prevalence in urban owned dogs. Drug resistance in Ancylostoma caninum is becoming a discussion point in small animal practices across the region. This study aimed to identify hookworm species present in Australian and New Zealand dogs using molecular techniques. The ITS-2 and isotype-1 β-tubulin assays were used to identify and quantify hookworm species. Results showed absence of coinfection in Australian samples from Greater Sydney region belonging either to A. caninum or Uncinaria stenocephala, while New Zealand samples were a mixture of A. caninum and U. stenocephala. The amplified isotype-1 β-tubulin sequences exhibited susceptibility to benzimidazole drugs. Rare mutations were identified in A. caninum and U. stenocephala sequences, representing a small percentage of reads. This study highlights the importance of molecular techniques in accurately identifying and quantifying hookworm species in dog populations.
钩虫（钩口科）因其对健康的影响和人畜共患病的潜在风险，在犬类中是广为人知的寄生虫。虽然粪便分析是传统的检测方法，但饲养管理和驱虫措施的改进已降低了其在城市家养犬中的流行率。犬钩虫的耐药性正成为该地区小动物诊疗中的一个讨论话题。本研究旨在利用分子技术鉴定澳大利亚和新西兰犬类体内存在的钩虫种类。采用内转录间隔区2（ITS-2）和1型β微管蛋白检测法来鉴定和定量钩虫种类。结果显示，来自大悉尼地区的澳大利亚样本中不存在犬钩虫或窄头钩口线虫的混合感染，而新西兰样本则是犬钩虫和窄头钩口线虫的混合体。扩增出的1型β微管蛋白序列显示对苯并咪唑类药物敏感。在犬钩虫和窄头钩口线虫序列中鉴定出罕见突变，但其在读取序列中所占比例较小。本研究强调了分子技术在准确鉴定和定量犬类群体中钩虫种类方面的重要性。
