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与环氧胺树脂封闭剂相比，硅酸钙基根管封闭剂对人根尖乳头干细胞的细胞相容性和细胞迁移评估：一项体外研究。

Cytocompatibility and cell migration evaluation of calcium silicate-based root canal sealer compared to epoxide-amine resin sealer in stem cells from human apical papilla: An in-vitro study.

作者信息

Nguyen Linh Cao Hoai, Pham Loc Nguyen Gia, Van Pham Khoa, Nguyen Minh Duc, Ngo Lan Thi Quynh

机构信息

Faculty of Odonto-Stomatology, University of Medicine and Pharmacy at Ho Chi Minh City, Ho Chi Minh City, Vietnam.

Odonto - Maxillo Facial Hospital of Ho Chi Minh City, Ho Chi Minh City, Vietnam.

出版信息

Aust Endod J. 2023 Dec;49(3):554-562. doi: 10.1111/aej.12786. Epub 2023 Aug 3.

DOI:10.1111/aej.12786
PMID:37537929
Abstract

The purpose of this study was to assess the effect of a calcium silicate-based sealers (CeraSeal) and an epoxy resin-based sealer (AH Plus) on cytotoxicity and cell migration of stem cell from the human apical papilla (hSCAPs) by using the Alamar Blue, Annexin V-FICT and wound healing assays. In Alamar Blue assay, hSCAPs exposed to undiluted CeraSeal extract had significantly higher cell viability compared with that observed when cells were treated with AH Plus in all experimental period (p < 0.001). The flow cytometry analysis confirmed the comparison on viable cells and indicated that AH Plus increased apoptosis compared to CeraSeal and the control groups (p < 0.001). Additionally, AH Plus exhibited significantly lower level of cell migration than CeraSeal and the control for up to 48 h observation (p < 0.01). In summary, calcium silicate-based sealer (CeraSeal) is less cytotoxic and more biocompatible than epoxy resin-based sealer (AH Plus).

摘要

本研究旨在通过使用alamar蓝、膜联蛋白V-FICT和伤口愈合试验，评估硅酸钙基封闭剂（CeraSeal）和环氧树脂基封闭剂（AH Plus）对人根尖乳头干细胞（hSCAPs）细胞毒性和细胞迁移的影响。在alamar蓝试验中，在所有实验期间，与用AH Plus处理细胞时相比，暴露于未稀释的CeraSeal提取物的hSCAPs具有显著更高的细胞活力（p＜0.001）。流式细胞术分析证实了活细胞的比较，并表明与CeraSeal和对照组相比，AH Plus增加了细胞凋亡（p＜0.001）。此外，在长达48小时的观察中，AH Plus的细胞迁移水平明显低于CeraSeal和对照组（p＜0.01）。总之，硅酸钙基封闭剂（CeraSeal）比环氧树脂基封闭剂（AH Plus）具有更低的细胞毒性和更高的生物相容性。

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