School and Hospital of Stomatology, Tianjin Medical University, 12 Observatory Road, Tianjin, 300070, China.
Tanggu Stomatological Hospital, No. 171; Yongjiu Street, Binhai New Area, Tianjin, 300450, China.
BMC Oral Health. 2024 Aug 30;24(1):1023. doi: 10.1186/s12903-024-04774-w.
Calcium silicate-based bioceramics have been applied in endodontics as advantageous materials for years, many chemical components and new synthesizing methods were used to improve the base formulation of the materials for positively affecting the sealers properties. Recently, a novel biomaterial formulation, grounded in strontium silicate, has been introduced to the market, offering potential advancements in the field.
To comparatively analyze the cytotoxicity and cell migration effects of a novel strontium silicate-based bioceramic material (CRoot SP) and those of calcium silicate-based (iRoot SP) and epoxide amine resin (AH Plus) sealers on stem cells derived from rat apical papilla(rSCAPs).
rSCAPs were isolated and characterized in vitro and subsequently cultured in the presence of various concentrations of CRoot SP, iRoot SP and AH Plus extracts. Cytotoxicity was assessed by CCK-8 assay, and cell-migration capacity was assessed by using wound healing assays .
No significant differences in cell viability were observed in the 0.02 mg/mL and 0.2 mg/mL sealer groups. The cell viability of CRoot SP was consistently greater than that of iRoot SP at concentrations of 5 mg/mL and 10 mg/mL across all time points. Maximum cytotoxic effect was noted on day 5 with 10 mg/mL AH Plus.The scratch was partly healed by cell migration in all groups at 24 h, and the 0.02 mg/mL, and 0.2 mg/mL CRoot SP exerted beneficial effects on rSCAPs migration.
CRoot SP exhibited less cytotoxic than the iRoot SP and AH Plus extracts after setting. A lower concentration of CRoot SP thus promotes the cell migration capacity of rSCAPs, and it may achieve better tissue repair during root canal treatment.
硅酸钙基生物陶瓷多年来一直被应用于牙髓学，因其作为有利材料，许多化学组分和新的合成方法被用于改进材料的基础配方，以积极影响密封剂的性能。最近，一种新型生物材料配方，以硅锶为基础，已被引入市场，为该领域提供了潜在的进步。
比较分析新型硅酸锶基生物陶瓷材料（CRoot SP）与硅酸钙基（iRoot SP）和环氧化物胺树脂（AH Plus）封剂对大鼠根尖乳头干细胞（rSCAPs）的细胞毒性和细胞迁移效应。
rSCAPs 在体外分离和鉴定，然后在含有不同浓度的 CRoot SP、iRoot SP 和 AH Plus 提取物的培养液中培养。通过 CCK-8 测定法评估细胞毒性，通过划痕愈合试验评估细胞迁移能力。
在 0.02 mg/mL 和 0.2 mg/mL 封剂组中，细胞活力没有显著差异。在所有时间点，5 mg/mL 和 10 mg/mL 浓度下，CRoot SP 的细胞活力始终大于 iRoot SP。在 10 mg/mL AH Plus 下，第 5 天观察到最大的细胞毒性效应。在 24 h 时，所有组的划痕均通过细胞迁移部分愈合，0.02 mg/mL 和 0.2 mg/mL 的 CRoot SP 对 rSCAPs 迁移有有益的影响。
凝固后，CRoot SP 的细胞毒性小于 iRoot SP 和 AH Plus 提取物。因此，较低浓度的 CRoot SP 可促进 rSCAPs 的细胞迁移能力，并且在根管治疗期间可能实现更好的组织修复。
